LOCUS       sequence-123590-        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  TITLE     de Lange Lab Vectors
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLPC-N MYC
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(853..882)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      882..891
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(922..941)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(923..941)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(986..1189)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1015..1035)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1190..1569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1741..1760
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1849..2448)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1932..1952)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2429..2448
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2466..2882)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2482..2498)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2514..2536)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3083..3282
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3347..3816
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3847..3951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3952..4020,4021..4812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4170..4189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4972..5560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5461..5480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5714..5731
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcggccctc tagatgcatg ctcgagcggc
      781 cgccagtgtg atggatatct gcagaattcc agcacactgg cggccgttac tagtggatcc
      841 tctctttctt aacaaatctt cttcagaaat caacttttgt tccatggtgg ctgatccgag
      901 ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag
      961 cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc
     1021 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     1081 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     1141 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     1201 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     1261 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     1321 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     1381 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     1441 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     1501 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     1561 atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     1621 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     1681 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     1741 cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat ccgatgcatg
     1801 gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg
     1861 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     1921 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     1981 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     2041 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     2101 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     2161 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     2221 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     2281 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     2341 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     2401 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagctgatc
     2461 cggccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa
     2521 cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg
     2581 tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg
     2641 taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg
     2701 gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat
     2761 tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg
     2821 atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt aacagtctgg
     2881 ccctaatttt cagacaaata cagaaacaca gtcagacaga gacaacacag aacgatgctg
     2941 cagcgctgca gcagacaaga cgcgcggctt cggttccaaa ccgaaagcaa aaattcagac
     3001 ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc tgacggggtc
     3061 ggattccaca tcgactccct tcctcaggtc gggccacaaa aacggccccc aaagtccctg
     3121 ggacgtctcc cagggttgcg gccgggtgtt cagaactcgt cagttccacc acgggtccgc
     3181 cagatacaga gctagttagc taactagtac cgacgcaggc gcataaaatc agtcatagac
     3241 actagacaat cggacagaca cagataagtt gctggccagc ttgcctcccg gtggtgggtc
     3301 ggtggtccct gggcaggggt ctcccgatcc cggacgagcc cccaaatgaa agacccccgc
     3361 tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac cacaagtcgg
     3421 atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag tcaatcggag
     3481 gactggcgcc ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc
     3541 gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca
     3601 ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga gggctggacc
     3661 gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt accacagata
     3721 tcctgtttgg cccatattca gctgttccat ctgttcttgg ccctgagccg gggcaggaac
     3781 tgcttaccac agatatcctg tttggcccat attcaggctg caggtggcac ttttcgggga
     3841 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3901 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4021 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4081 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4141 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4201 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4261 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4321 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4381 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4441 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4501 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4561 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4621 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4681 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4741 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4801 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttgcggccg
     4861 caaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
