LOCUS       sequence-123592-        7539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AdenoAssociated Virus plasmid containing expanded repeats--CTG 700x 
            that is the mutation found in Myotonic Dystrophic type 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7539)
  TITLE     Charlet-Berguerand plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV CTG 700x
FEATURES             Location/Qualifiers
     source          1..7539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        206..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     710..734
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..1240
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     regulatory      1318..1327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1324..1326,1327..1329,1330..2040)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1324..1326,1327..1329,1330..2040)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1369..1390)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1630..1649)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1977..1998
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4286..4762
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4485..4504)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4802..4942
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4802..4931
                     /label=AAV2 ITR
     rep_origin      5017..5472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5104..5123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5314..5335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5489..5508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5608..5630
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5668..5686)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5754..5858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5859..5927,5928..6719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6077..6096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6890..7478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7379..7398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      721 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      781 gggaccgatc cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg
      841 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat
      901 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
      961 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
     1021 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
     1081 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
     1141 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
     1201 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
     1261 ctgtgtgctg gcccatcact ttggcaaaga attgggattc gaacatcgat tgaattcgcc
     1321 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1381 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1441 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1501 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1561 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1621 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1681 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1741 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1801 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1861 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1921 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1981 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2041 aagctagcgt ttaaacgggc cctctagact cgagtcccac gcgggccatt tccacgcggg
     2101 ccatttgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2161 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2221 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2281 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2341 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2401 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2461 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2521 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2581 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2641 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2701 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2761 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2821 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2881 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2941 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3001 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3061 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3121 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3181 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3241 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3301 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3361 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3421 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3481 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3541 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3601 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3661 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3721 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3781 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3841 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3901 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3961 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     4021 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     4081 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     4141 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     4201 gctgaaatgg aaatgggacg catgcgaatt cggatccgag ctcggtacca agcttgcctc
     4261 gagcagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     4321 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     4381 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     4441 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     4501 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     4561 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     4621 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     4681 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     4741 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     4801 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4861 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4921 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4981 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     5041 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5101 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5161 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5221 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5281 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5341 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     5401 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5461 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5521 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5581 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5641 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5701 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7021 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7501 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
