LOCUS       sequence-12360-e        9503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-VP16AD
FEATURES             Location/Qualifiers
     source          1..9503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1130..1150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1163..1396
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     protein_bind    complement(1881..1914)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2099..2117)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2569..2640,3042..3315,3390..4044,4106..4421,4625..4756,
                     4827..5441)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(6215..6248)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6272..6305)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(6535..6736,6737..6767)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7304..7323)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7423..7445
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7483..7501)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7569..7673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7674..7742,7743..8534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7892..7911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8705..9293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9194..9213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccgcgg ccgcaaaaca tggataaagc ggaattaatt cccgagcctc caaaaaagaa
     1141 gagaaaggtc gaattgggta ccgccccccc gaccgatgtc agcctggggg acgagctcca
     1201 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     1261 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     1321 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     1381 tgacgagtac ggtggggcta gcggaggagg tggtggaggt ggaggtggag gtactagtct
     1441 ggagatcgag gccgccttcc tggagcgcga gaacaccgcc ctggagaccc gcgtggccga
     1501 gctgcgccag cgcgtgcagc gcctgcgcaa ccgcgtgagc cagaaccgca cccgctacgg
     1561 ccccctgggc ggcggcaagt aaggcgcgcc tctagatcga taccgtcgac taaagccaaa
     1621 tagaaaatta ttcagttcct ggcttaagtt tttaaaagtg atattattta tttggttgta
     1681 accaaccaaa agaatgtaaa taactaatac ataattatgt tagttttaag ttagcaacaa
     1741 attgatttta gctatattag ctacttggtt aataaataga atatatttat ttaaagataa
     1801 ttgcgttttt attgtcaggg agtgagtttg cttaaaaact cgtttagatc cactagctta
     1861 attaagcatg cctaggctag ataacttcgt atagcataca ttatacgaag ttatagcttc
     1921 tgatggaatt agaacttggc aaaacaatac tgagaatgaa gtgtatgtgg aacagaggct
     1981 gctgatctcg ttcttcaggc tatgaaactg acacatttgg aaaccacagt acttagaacc
     2041 acaaagtggg aatcaagaga aaaacaatga tcccacgaga gatcgaattc tagtatgtat
     2101 gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac
     2161 tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     2221 ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     2281 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     2341 ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat
     2401 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     2461 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
     2521 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
     2581 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
     2641 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
     2701 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac
     2761 aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt
     2821 tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt
     2881 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
     2941 aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca
     3001 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
     3061 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac
     3121 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
     3181 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
     3241 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
     3301 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
     3361 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
     3421 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
     3481 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
     3541 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
     3601 ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct
     3661 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
     3721 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
     3781 gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga
     3841 gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct
     3901 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
     3961 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
     4021 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt
     4081 acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
     4141 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
     4201 ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt
     4261 tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca
     4321 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
     4381 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
     4441 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg
     4501 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
     4561 agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt
     4621 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
     4681 tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
     4741 ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa
     4801 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
     4861 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
     4921 tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
     4981 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
     5041 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
     5101 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
     5161 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
     5221 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
     5281 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
     5341 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
     5401 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     5461 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
     5521 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
     5581 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
     5641 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
     5701 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
     5761 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
     5821 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt
     5881 tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta
     5941 tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta atttaatatt
     6001 atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac
     6061 tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat
     6121 aatagtgttg tttatatata tcaaaaataa caactataat aataagaata catttaattt
     6181 agaaaatgct tggatttcac tggaactaga attcataact tcgtatagca tacattatac
     6241 gaagttatgg atctggccgc gctcttccgc tgaagttcct atactttcta gagaatagga
     6301 acttcctcga cggtagcggt ccggttgttt tcgtgctcat cgcgagtacg tatttatttt
     6361 taaaaaataa taaacgcggc aaacacaatc acacaaatgt gcaggcacag tgggacaaag
     6421 tgcgctaaat ttggatatgt aataaatcca aaaccaaaaa gaaaacaaaa aacggtaaaa
     6481 tattttactg ttgttaaaat tcgatcattc attattcgct gcatgaatta gctttgcgta
     6541 ctcgcaaatt attaaaaata aaactttaaa aataatttcg tctaattaat attatgagtt
     6601 aattcaaacc ccacggacat gctaagggtt aatcaacaat catatcgctg tctcactcag
     6661 actcaatacg acactcagaa tactattcct ttcactcgca cttattgcaa gcatacgtta
     6721 agtggatgtc tcttgccgac gggaccacct tatgttattt catcatggtc tggccattct
     6781 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     6841 gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat taccatcgtg
     6901 tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa
     6961 gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga
     7021 tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc
     7081 gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct
     7141 tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc
     7201 tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg
     7261 cgattcggtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     7321 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7381 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7441 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7501 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8821 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9061 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     9121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct
     9301 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
     9361 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
     9421 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
     9481 ttaacttctc cgccgccgat ctg
//
