LOCUS       sequence-123606-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_N300S
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..60)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
       61 ctcgaggcta ccaacaggtt ttttcagcag atgtttcagg gtggtggtaa caccattatc
      121 acgcaggcta ccataacaac gttcaaatgc ggcaacaaat tccggactat tcggaattgc
      181 ggtgccaaaa atttcttcaa ctgccagcag acgctggcta atcagtgcat catctgaaac
      241 cagaccctga caaaattctg cacgcggatc aggaatggta tagctcacac cattttcatc
      301 aacgcctttc agatacagtg cccatgctgc aacaaccagt gctgcacgtt cggtttcacg
      361 accatctgca atcagacgat taattgtcgg aacggtaaat ttaggaaatt tgctgctacc
      421 atcactacaa acacgttcca gctgatctgc aattgcctga ttgctaaaac gatcaaccag
      481 ggtctgttta taatcggtca gatcaatacc cggaaccgga gccagattcg gggtcacatc
      541 cagatccata tatgcacgca tataggcaac aaacagcgga tcgttcatgg tttcatgcac
      601 aaaacgataa cctttcagaa aacccagata ggtcagtgcc agatggctac cgctcagcag
      661 accaattttc atttcttcat acggggtaac atcatcggta aactgaacac caactttttc
      721 ccatgccgga cgaccattaa caaatttatc ttccagaacc cactgaacaa acggttcaca
      781 aacaacaggc catgcatcat caataccatg ttcgtcatgc agttgcagac gatgtgcggt
      841 gctggtcatc ggggtaatac gatcaaccat tgcattcgga aagctaacat gggctttaat
      901 ccaatcatgc agttctgcat tatgcagggc tgcaaatgcc agcagggctt tacgggtaac
      961 tgcaccatta tgcggcaggt tatcacagct cata
//
