LOCUS       sequence-123608-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNALys scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSK
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(252..271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(253..271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(278..295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(278..294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(287..309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(523..542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagctggtac cgggcccccc ctcgaggtcg ccccatcaaa aaaatattct caacataaaa
       61 aactttgtgt aatacttgta acgctgaatt cgcccggata gctcagtcgg tagagcagcg
      121 gccgatatcc gcgggtccag ggttcaagtc cctgttcggg cgccactgca gatccttagc
      181 gaaagctaag gatttttttt aagcttgatg ggggcttagg atccggagct ccaagcttta
      241 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      301 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      361 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      421 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      481 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      541 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      601 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      661 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttgga
//
