LOCUS       sequence-123610-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNAMet scaffold with the streptavidin aptamer tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSMrnaStrp
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(320..339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(321..339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(346..363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(346..362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(355..377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(591..610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaagctggta ccgggccccc cctcgaggtc gccccatcaa aaaaatattc tcaacataaa
       61 aaactttgtg taatacttgt aacgctgaat tcggctacgt agctcagttg gttagagcag
      121 cggccgaact cgaccagaat catgcaagtg cgtaagatag tcgcgggtcg agtcgacggt
      181 gacgtcgaac aagcggccgc gggtcacagg ttcgaatccc gtcgtagcca ccactgcaga
      241 tccttagcga aagctaagga ttttttttaa gcttgatggg ggcttaggat ccggagctcc
      301 aagctttagc tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
      361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      481 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      541 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      601 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      661 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      721 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      781 ggagtccacg ttctttaata gtggactctt gttccaaa
//
