LOCUS       sequence-123611-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNAMet scaffold with the streptavidin aptamer tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSMrnaStrp
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(311..329)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(350..366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(363..385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    374..390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(398..404,405..422,423..428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    443..464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(581..598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(832..851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggagc tccggatcct aagcccccat caagcttaaa aaaaatcctt agctttcgct
       61 aaggatctgc agtggtggct acgacgggat tcgaacctgt gacccgcggc cgcttgttcg
      121 acgtcaccgt cgactcgacc cgcgactatc ttacgcactt gcatgattct ggtcgagttc
      181 ggccgctgct ctaaccaact gagctacgta gccgaattca gcgttacaag tattacacaa
      241 agttttttat gttgagaata tttttttgat ggggcgacct cgaggggggg cccggtacca
      301 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
      361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      901 gatacctgtc cgcct
//
