LOCUS       sequence-123613-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  orange luminescent protein for high-speed single-cell and whole body
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Orange Nano-lantern/pRSETB
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..100
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(104..112,113..127)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             134..787
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             134..787
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca
       61 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      121 cgataaggat ccgatggtga gcgtgatcaa gcccgagatg aagatgaggt actacatgga
      181 cggctccgtc aatgggcatg agttcacaat cgagggtgag ggcacaggca gaccttacga
      241 gggacatcag gagatgacac tgcgcgtcac aatggccgag ggcgggccaa tgcctttcgc
      301 cttcgacctg gtgtcccacg tgttctgtta cggccacaga gtttttacta aatatccaga
      361 agagatccca gactatttca agcaggcctt tcctgagggc ctgtcctggg agaggtccct
      421 ggagttcgag gacggcggct ccgcctccgt gagcgcccac atcagcctga ggggcaacac
      481 cttctaccac aagtccaagt tcaccggcgt gaacttcccc gccgacggcc ccatcatgca
      541 gaaccagagc gtggactggg agccctccac cgagaagatc accgccagcg acggcgtgct
      601 gaagggcgac gtgaccatgt acctgaagct ggagggcggc ggcaaccaca agtgccagat
      661 gaagaccacc tacaaggccg ccaaggagat cctggagatg cccggcgacc actacatcgg
      721 ccacaggctg gtgaggaaga ccgagggcaa catcaccgag caggtggagg acgccgtggc
      781 ccactccggt accaaggtgt acgaccccga gcaacgcaaa cgcatgatca ctgggcctca
      841 gtggtgggct
//
