LOCUS       sequence-123615-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNALys scaffold with the sephadex aptamer tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSKrnaSeph
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(289..308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(290..308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(315..332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(324..346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(560..579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aacaaaagct ggtaccgggc cccccctcga ggtcgcccca tcaaaaaaat attctcaaca
       61 taaaaaactt tgtgtaatac ttgtaacgct gaattcgccc ggatagctca gtcggtagag
      121 cagcggccga gtaatttacg tcgacggtga cgtcgatggt tgcggccgcg ggtccagggt
      181 tcaagtccct gttcgggcgc cactgcagat ccttagcgaa agctaaggat tttttttaag
      241 cttgatgggg gcttaggatc cggagctcca agctttagct ccaattcgcc ctatagtgag
      301 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
      481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tg
//
