LOCUS       sequence-123617-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU1
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(101..129)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(469..502)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        506..553
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gggcgattgg gccctctgat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      121 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      181 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      241 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaacact
      301 tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg
      361 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      421 caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacga agttcctata
      481 ctttctagag aataggaact tccctgttga caattaatca tcggcatagt atatcggcat
      541 agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgagt ctgaaagaaa
      601 aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc
      661 ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct
      721 gcgcgattga ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg
      781 tgatggcagc cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg
      841 cacatgaaaa ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc
      901 gtaacaacag cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg
      961 ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc
     1021 atctgccgct ggacggcgca caa
//
