LOCUS       sequence-123618-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU1
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(127..147)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    556..589
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     598..614
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     598..614
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
       61 ccgccagtgt gctggaattc gccctttttg tatagttcat ccatgccatg tgtaatccca
      121 gcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa
      181 agggcagatt gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
      241 ggagtatttt gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtgtcta
      301 attttgaagt tctgaaaatt taaatcagat tagttagtta gtaaaatcat gtttaaactt
      361 acaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag
      421 ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac
      481 tcgattctat taacaagggt atcaccttca aacttgactt cagcacctga aaatttaaat
      541 atgtatggtt agttagaagt tcctattctc tagaaagtat aggaacttcg cggccgctct
      601 agaactagtg gatcctcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt
      661 ctctttaata cctgtttttg cttcatattg ttcagcgaca gcttgctgta cggcaggcac
      721 cagctcttcc gggatcagcg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac
      781 gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac
      841 ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact cgcccatacg
      901 tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag
      961 tatatgacgc acgcgttttg ccacgatcgg gtccagttca tgcgcaacag cgttgaactc
     1021 ttcaatggtg acatcacgca gggctggctg ctggaagaaa cgcgcaccgg tttcgcactg
     1081 ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagt
//
