LOCUS       sequence-12362-e       11213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBMPGAL4LWL
FEATURES             Location/Qualifiers
     source          1..11213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1096..1195
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             1458..4103
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(4599..4615)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4599..4615)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(4633..4666)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4851..4869)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5321..5392,5794..6067,6142..6796,6858..7173,7377..7508,
                     7579..8193)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(8967..9000)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9025..9045)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9025..9043)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9064..9080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9064..9080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9077..9099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9112..9118,9119..9136,9137..9142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9157..9178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9295..9312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9466..10054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9546..9565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10225..11016,11017..11085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10848..10867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11086..11190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
      721 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
      781 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
      841 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
      901 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg aggtcgacgg
      961 tatcgataag cttgcggccg cggcgcgcca gatcttaatt aagctagttg caggaattcg
     1021 gcttcgacta gtactgacgg acacaccgaa gccccggcgg caaccctcag cggatgcccc
     1081 ggggcttcac gttttcccag gtcagaagcg gttttcggga gtagtgcccc aactggggta
     1141 acctttgagt tctctcagtt gggggcgtag ggtcgccgac atgacacaag gggttgtgac
     1201 cggggtggac acgtacgcgg gtgcttacga ccgtcagtcg cgcgagcgcg actagtacaa
     1261 gccgaattcg atatcaagct taccgaagta tacacttaaa ttcagtgcac gtttgcttgt
     1321 tgagaggaaa ggttgtgtgc ggacgaattt ttttttgaaa acattaaccc ttacgtggat
     1381 cccgtaagca aataaacaag cgcagctgaa caagctaaac aatctgcagc ccaagcttga
     1441 agcaagcctc ctgaaagatg aagctactgt cttctatcga acaagcatgc gatatttgcc
     1501 gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca
     1561 actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga
     1621 cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag
     1681 aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag
     1741 gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg
     1801 agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag
     1861 agagtagtaa caaaggtcaa agacagttga ctgtatcgat tgactcggca gctcatcatg
     1921 ataactccac aattccgttg gattttatgc ccagggatgc tcttcatgga tttgattggt
     1981 ctgaagagga tgacatgtcg gatggcttgc ccttcctgaa aacggacccc aacaataatg
     2041 ggttctttgg cgacggttct ctcttatgta ttcttcgatc tattggcttt aaaccggaaa
     2101 attacacgaa ctctaacgtt aacaggctcc cgaccatgat tacggataga tacacgttgg
     2161 cttctagatc cacaacatcc cgtttacttc aaagttatct caataatttt cacccctact
     2221 gccctatcgt gcactcaccg acgctaatga tgttgtataa taaccagatt gaaatcgcgt
     2281 cgaaggatca atggcaaatc ctttttaact gcatattagc cattggagcc tggtgtatag
     2341 agggggaatc tactgatata gatgtttttt actatcaaaa tgctaaatct catttgacga
     2401 gcaaggtctt cgagtcaggt tccataattt tggtgacagc cctacatctt ctgtcgcgat
     2461 atacacagtg gaggcagaaa acaaatacta gctataattt tcacagcttt tccataagaa
     2521 tggccatatc attgggcttg aatagggacc tcccctcgtc cttcagtgat agcagcattc
     2581 tggaacaaag acgccgaatt tggtggtctg tctactcttg ggagatccaa ttgtccctgc
     2641 tttatggtcg atccatccag ctttctcaga atacaatctc cttcccttct tctgtcgacg
     2701 atgtgcagcg taccacaaca ggtcccacca tatatcatgg catcattgaa acagcaaggc
     2761 tcttacaagt tttcacaaaa atctatgaac tagacaaaac agtaactgca gaaaaaagtc
     2821 ctatatgtgc aaaaaaatgc ttgatgattt gtaatgagat tgaggaggtt tcgagacagg
     2881 caccaaagtt tttacaaatg gatatttcca ccaccgctct aaccaatttg ttgaaggaac
     2941 acccttggct atcctttaca agattcgaac tgaagtggaa acagttgtct cttatcattt
     3001 atgtattaag agattttttc actaatttta cccagaaaaa gtcacaacta gaacaggatc
     3061 aaaatgatca tcaaagttat gaagttaaac gatgctccat catgttaagc gatgcagcac
     3121 aaagaactgt tatgtctgta agtagctata tggacaatca taatgtcacc ccatattttg
     3181 cctggaattg ttcttattac ttgttcaatg cagtcctagt acccataaag actctactct
     3241 caaactcaaa atcgaatgct gagaataacg agaccgcaca attattacaa caaattaaca
     3301 ctgttctgat gctattaaaa aaactggcca cttttaaaat ccagacttgt gaaaaataca
     3361 ttcaagtact ggaagaggta tgtgcgccgt ttctgttatc acagtgtgca atcccattac
     3421 cgcatatcag ttataacaat agtaatggta gcgccattaa aaatattgtc ggttctgcaa
     3481 ctatcgccca ataccctact cttccggagg aaaatgtcaa caatatcagt gttaaatatg
     3541 tttctcctgg ctcagtaggg ccttcacctg tgccattgaa atcaggagca agtttcagtg
     3601 atctagtcaa gctgttatct aaccgtccac cctctcgtaa ctctccagtg acaataccaa
     3661 gaagcacacc ttcgcatcgc tcagtcacgc cttttctagg gcaacagcaa cagctgcaat
     3721 cattagtgcc actgaccccg tctgctttgt ttggtggcgc caattttaat caaagtggga
     3781 atattgctga tagctcattg tccttcactt tcactaacag tagcaacggt ccgaacctca
     3841 taacaactca aacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg
     3901 ataacttcat gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac
     3961 cactgtcacc tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga
     4021 tgtttaatac cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc
     4081 caccaaaccc aaaaaaagag taaaatgaat cgtagatact gaaaaacccc gcaagttcac
     4141 ttcaactgtg catcgtgcac catctcaatt tctttcattt atacatcgtt ttgccttctt
     4201 ttatgtaact atactcctct aagtttcaat cttggccatg taacctctga tctatagaat
     4261 tttttaaatg actagaatta atgcccatct tttttttgga cctaaattct tcatgaaaat
     4321 atattacgag ggcttattca gaagcttatc gataccgtcg actaaagcca aatagaaaat
     4381 tattcagttc ctggcttaag tttttaaaag tgatattatt tatttggttg taaccaacca
     4441 aaagaatgta aataactaat acataattat gttagtttta agttagcaac aaattgattt
     4501 tagctatatt agctacttgg ttaataaata gaatatattt atttaaagat aattgcgttt
     4561 ttattgtcag ggagtgagtt tgcttaaaaa ctcgtttaga tccactagtt ctagagcggc
     4621 cggccgctct agataacttc gtatagcata cattatacga agttatagct tctgatggaa
     4681 ttagaacttg gcaaaacaat actgagaatg aagtgtatgt ggaacagagg ctgctgatct
     4741 cgttcttcag gctatgaaac tgacacattt ggaaaccaca gtacttagaa ccacaaagtg
     4801 ggaatcaaga gaaaaacaat gatcccacga gagatcgaat tctagtatgt atgtaagtta
     4861 ataaaaccca tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg
     4921 atatcattga acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta
     4981 ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt
     5041 gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct
     5101 ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag
     5161 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     5221 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     5281 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     5341 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     5401 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     5461 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat
     5521 agtgacacga aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat
     5581 catatcatga tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa
     5641 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     5701 acctacttcg taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt
     5761 gtagttgata tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc
     5821 tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg
     5881 ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac
     5941 aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac
     6001 cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc
     6061 aagaacggtg agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc
     6121 aattaccaat ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga
     6181 tgggcagttc cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc
     6241 agggcatcca agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca
     6301 aggagatgca ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa
     6361 cggccaggga acacctgatt ttccaagcca tggtgcggat gccacgacat ctgacctatc
     6421 ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc
     6481 acacgatcat cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc
     6541 tggcattcgc ctccgaggct ctaaccgatc cgccgcttct gatctgcgat gagcccacct
     6601 ccggactgga ctcctttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga
     6661 agggcaagac cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg
     6721 acaagatcct tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg
     6781 tcgacttctt ttcctagtga gttcgatgtg tttattaagg gtatctagta ttacataaca
     6841 tctcaactcc tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt
     6901 tacgtacagg tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc
     6961 aagatatgcg acaattttgc cattagcaaa gtagcccggg atatggagca gttgttggcc
     7021 accaaaaatc tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc
     7081 tggttcatgc agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca
     7141 ctcctcgtaa aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga
     7201 tataacgctt acaattcttg gaaacaaatt cgctagattt tagatagaat tgcctgattc
     7261 cacacccttc ttagtttttt tcaatgagat gtatagttta tagttttgca gaagataaat
     7321 aaatttcatt taactcgcga atattaatga gatgcgagta acattttaat ttgcagatgg
     7381 ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg ggtgtgatga
     7441 atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca
     7501 cgataaatgt aagtcatgtt tagaatacat ttgcatttca ataatttact aactttctaa
     7561 tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga
     7621 agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga attgccgctt
     7681 tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc
     7741 ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg
     7801 tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt
     7861 ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg
     7921 ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg
     7981 ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc
     8041 acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg
     8101 ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg
     8161 gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg
     8221 tttttttttt ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta
     8281 atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc
     8341 cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc
     8401 tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta
     8461 tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc
     8521 gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac
     8581 gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg
     8641 gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc
     8701 aatatatttg tatatatttc cctcacagct atatttattc taatttaata ttatgacttt
     8761 ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg
     8821 acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt
     8881 tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg
     8941 cttggatttc actggaacta gaattcataa cttcgtatag catacattat acgaagttat
     9001 ggatctgagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     9061 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9241 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9661 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9901 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
