LOCUS       sequence-123620-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of yfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU2
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(100..128)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(468..501)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        505..552
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gggcgatggg ccctctgatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgccctta tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      121 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      181 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      241 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gccaacactt
      301 gtcactactt tcggttatgg tcttcaatgc ttcgccagat acccagatca tatgaaacag
      361 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      421 aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtacgaa gttcctatac
      481 tttctagaga ataggaactt ccctgttgac aattaatcat cggcatagta tatcggcata
      541 gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgagtc tgaaagaaaa
      601 aacacaatct ctgtttgcca acgcatttgg ctaccctgcc actcacacca ttcaggcgcc
      661 tggccgcgtg aatttgattg gtgaacacac cgactacaac gacggtttcg ttctgccctg
      721 cgcgattgat tatcaaaccg tgatcagttg tgcaccacgc gatgaccgta aagttcgcgt
      781 gatggcagcc gattatgaaa atcagctcga cgagttttcc ctcgatgcgc ccattgtcgc
      841 acatgaaaac tatcaatggg ctaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg
      901 taacaacagc ttcggcggcg tggacatggt gatcagcggc aatgtgccgc agggtgccgg
      961 gttaagttct tccgcttcac tggaagtcgc ggtcggaacc gtattgcagc agctttatca
     1021 tctgccgctg gacggcgcac aaatcgcgct taacggtcag gaagcagaaa ac
//
