LOCUS       sequence-123621-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of yfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU2
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(125..145)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    554..587
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     596..612
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     596..612
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
       61 ccgccagtgt gctggaattc gccctttgta tagttcatcc atgccatgtg taatcccagc
      121 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
      181 ggcagattgg taggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
      241 agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat
      301 tttgaagttc tgaaaattta aatcagatta gttagttagt aaaatcatgt ttaaacttac
      361 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
      421 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
      481 gattctatta acaagggtat caccttcaaa cttgacttca gcacctgaaa atttaaatat
      541 gtatggttag ttagaagttc ctattctcta gaaagtatag gaacttcgcg gccgctctag
      601 aactagtgga tcctcagcac tgtcctgctc cttgtgatgg tttacaaacg taaaaagtct
      661 ctttaatacc tgtttttgct tcatattgtt cagcgacagc ttgctgtacg gcaggcacca
      721 gctcttccgg gatcagcgcg acgatacagc cgccaaatcc gccgccggtc atgcgtacgc
      781 cacctttgtc gccaatcaca gctttgacga tttctaccag agtgtcaatt tgcggcacgg
      841 tgatttcgaa atcatcgcgc atagaggcat gagactccgc catcaactcg cccatacgtt
      901 tcaggtcgcc ttgctccagc gcgctggcag cttcaacggt gcgggcgttt tcagtcagta
      961 tatgacgcac gcgttttgcc acgatcgggt ccagttcatg cgcaacagcg ttgaactctt
     1021 caatggtgac atcacgcagg gctggctgct ggaagaaacg cgcaccggtt tcgcactgtt
     1081 cacgacg
//
