LOCUS       sequence-12363-e        9510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Hanna J, Cheng AW, Saha K, Kim J, Lengner CJ, Soldner F, Cassady JP,
            Muffat J, Carey BW, Jaenisch R
  TITLE     Human embryonic stem cells with biological and epigenetic 
            characteristics similar to those of mouse ESCs.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
  PUBMED    20442331
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 May 18. 107(20):9222-7."
COMMENT     Sequence Label: pBRPyCAG-fmKlf4-DsRed-Ip
FEATURES             Location/Qualifiers
     source          1..9510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    576..710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        724..1107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1112..1389
                     /label=chicken beta-actin promoter
     intron          1390..2406
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2330..2352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2414..2433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2467..2500)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3973..4006)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4012..4689
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4142..4160)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4192..4212)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4410..4429
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4604..4627
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(4730..5304,5305..5307,5308..5316)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4897..4914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5124..5143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5317..5916
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5317..5336)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5813..5833
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5997..6014)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6003..6227
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6274..6290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6287..6309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6298..6314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6322..6328,6329..6346,6347..6352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6447..6646
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6493..6632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6652..6786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             7255..7446
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7440..7462)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7548..7688
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7603..7622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7703..7720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7874..8462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7954..7973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8633..9424,9425..9493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9256..9275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccgctttg gaaaaacaaa gactgtattt cctggaaatt aatgtttatt caataaactg
       61 tgtattcagc tatattcaca tagtggtgag gctgaaatga ggcgggaaga ggcggttggg
      121 gcttaattat atcaatttgg gtggccccac agcgcctcca aggcgccagt cctgttttga
      181 caagttgcct ctggaagcct ctcttctttt tctccagagt aagcggaggc caggggcccc
      241 cggcctctgc ttaatactaa aaaaaacagc tgttgtcata gtaatgattg ggtggaaaca
      301 ttccaggcct gggtggagag gctttttgct tcctcttgca aaaccacact gacattccag
      361 gcctgggtgg agaggctttt tgcttcctct tgcaaaacca cactgccctc tggagggcag
      421 ttgcctagca actaattaaa agaggatgtc gcacggccag ctgcggtcag ttagtcactt
      481 cctgcttaac tgacttgaca ttttctattt taagagtcgg gaggaaaatt actgtgttgg
      541 aggccctccg ccatcttctg aagctgaatc gaattaactt gtttattgca gcttataatg
      601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatatcatc
      721 gtcgacattg attattgact agctagttat taatagtaat caattacggg gtcattagtt
      781 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      841 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      901 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      961 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1021 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1081 tacgtattag tcatcgctat taccatgatg gtcgaggtga gccccacgtt ctgcttcact
     1141 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1201 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1261 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1321 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1381 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1441 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1501 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1561 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1621 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     1681 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1741 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1801 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1861 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1921 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1981 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2041 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2101 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2161 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2221 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2281 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2341 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2401 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     2461 ctcgacataa cttcgtatag catacattat acgaagttat ctcgagtctg acatggctgt
     2521 cagcgacgct ctgctcccgt ccttctccac gttcgcgtcc ggcccggcgg gaagggagaa
     2581 gacactgcgt ccagcaggtg ccccgactaa ccgttggcgt gaggaactct ctcacatgaa
     2641 gcgacttccc ccacttcccg gccgccccta cgacctggcg gcgacggtgg ccacagacct
     2701 ggagagtggc ggagctggtg cagcttgcag cagtaacaac ccggccctcc tagcccggag
     2761 ggagaccgag gagttcaacg acctcctgga cctagacttt atcctttcca actcgctaac
     2821 ccaccaggaa tcggtggccg ccaccgtgac cacctcggcg tcagcttcat cctcgtcttc
     2881 cccagcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcagct atccgatccg
     2941 ggccgggggt gacccgggcg tggctgccag caacacaggt ggatggctcc tctacagccg
     3001 agaatctgcg ccacctccca cggccccctt caacctggcg gacatcaatg acgtgagccc
     3061 ctcgggcggc ttcgtggctg agctcctgcg gccggagttg gacccagtat acattccgcc
     3121 acagcagcct cagccgccag gtggcgggct gatgggcaag tttgtgctga aggcgtctct
     3181 gaccacccct ggcagcgagt acagcagccc ttcggtcatc agtgttagca aaggaagccc
     3241 agacggcagc caccccgtgg tagtggcgcc ctacagcggt ggcccgccgc gcatgtgccc
     3301 caagattaag caagaggcgg tcccgtccta cacggtcagc cggtccctag aggcccattt
     3361 gagcgctgga ccccagctca gcaacggcca ccggcccaac acacacgact tccccctggg
     3421 gcggcagctc cccaccagga ctacccctac actgagtccc gaggaactgc tgaacagcag
     3481 ggactgtcac cctggcctgc ctcttccccc aggattccat ccccatccgg ggcccaacta
     3541 ccctcctttc ctgccagacc agatgcagtc acaagtcccc tctctccatt atcaagagct
     3601 catgccaccg ggttcctgcc tgccagagga gcccaagcca aagaggggaa gaaggtcgtg
     3661 gccccggaaa agaacagcca cccacacttg tgactatgca ggctgtggca aaacctatac
     3721 caagagttct catctcaagg cacacctgcg aactcacaca ggcgagaaac cttaccactg
     3781 tgactgggac ggctgtgggt ggaaattcgc ccgctccgat gaactgacca ggcactaccg
     3841 caaacacaca gggcaccggc cctttcagtg ccagaagtgc gacagggcct tttccaggtc
     3901 ggaccacctt gccttacaca tgaagaggca cttttaaatc ccacgtagtg cggccgcact
     3961 agttacttag gtataacttc gtatagcata cattatacga agttatccac catggcctcc
     4021 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
     4081 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     4141 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     4201 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
     4261 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     4321 gtggtgaccg tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag
     4381 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg
     4441 gagccctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
     4501 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
     4561 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
     4621 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
     4681 ttcctgtaga cggccgctcg ataagcttga tatcgaattc cgcccccccc ccctctccct
     4741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     4801 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5281 tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg
     5341 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5401 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5461 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5521 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5581 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5641 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5701 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5761 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5821 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5881 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     5941 ccgaccgaaa ggagcgcacg accccatgca tcgatgatct agagctcgct gatcagcctc
     6001 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6061 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6121 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6181 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6241 aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6421 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     6481 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatgca
     6541 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6601 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     6661 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6721 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6781 tggatcccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     6841 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     6901 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     6961 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     7021 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     7081 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     7141 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     7201 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     7261 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     7321 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     7381 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7441 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7501 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     7561 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     7621 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7681 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8281 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8581 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8761 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8821 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8881 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8941 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9001 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9061 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9121 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9181 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9241 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9301 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9361 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9421 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9481 gttgaatact catactcttc ctttttcaat
//
