LOCUS       sequence-123630-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kohinoor for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor/pRSETb
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..105
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(109..117,118..132)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             142..816
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 ctctagaata ttttgtttac tttagaagga gatatacata tgcggggttc tcatcatcat
       61 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      121 gatgacgata aggatcccac catgagtgtg attaaaccag acatgaagat caagctgcgt
      181 atggaaggcg ctgtaaatgg acacccgttc gcgattgaag gagttggcct tgggaagcct
      241 ttcgagggaa aacagagtat ggaccttaaa gtcaaagaag gcggacctct gcctttcgcc
      301 tatgacatct tgacaatggc cttctgttac ggcaacaggg tattcgccaa atacccagaa
      361 aatatagtag attatttcaa gcagtcgttt cctgagggct actcttggga acgaagcatg
      421 atttacgaag acgggggcat ttgtatcgcg acaaacgaca taaccctgga tggtgactgt
      481 tatatctatg aaattcgatt tgatggtgtg aactttcctg ccaatggtcc agttatgcag
      541 aagaggactg tgaaatggga gccatccact gagaaattgt atgtgcgtga tggagtgctg
      601 aagagcgatg gcaactacgc tctgtcgctt gaaggaggtg gccactaccg atgtgactcc
      661 aaaactactt ataaagctaa gaaggttgtc cagttgccag actatcacga tgtggtccac
      721 cacattgaga ttaaaagcca cgacagagat tacagtaatg ttaatctgca tgagcatgcc
      781 gaagcgcatt ctgggctgcc gaggcaggcc aagtaagaat tcgaagcttg atccggctgc
      841 taacaaagcc cgaaaggaag ctgagttggc
//
