LOCUS       sequence-123632-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the tRNAMet scaffold with the sephadex aptamer tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
  TITLE     A generic protocol for the expression and purification of 
            recombinant RNA in Escherichia coli using a tRNA scaffold.
  JOURNAL   Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 
            May 28.
  PUBMED    19478810
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2009"; volume: "4(6):947-59. doi"; pages: " 
            10.1038/nprot.2009.67. Epub 2009 May 28"
COMMENT     Sequence Label: pBSMrnaSeph
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(286..305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(287..305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(312..329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(312..328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(321..343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(557..576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaagctggta ccgggccccc cctcgaggtc gccccatcaa aaaaatattc tcaacataaa
       61 aaactttgtg taatacttgt aacgctgaat tcggctacgt agctcagttg gttagagcag
      121 cggccgagta atttacgtcg acggtgacgt cgatggttgc ggccgcgggt cacaggttcg
      181 aatcccgtcg tagccaccac tgcagatcct tagcgaaagc taaggatttt ttttaagctt
      241 gatgggggct taggatccgg agctccaagc tttagctcca attcgcccta tagtgagtcg
      301 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
      481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      601 tttccccgtc aagctctaaa tcgggggctc cctttaaggt tccgattta
//
