LOCUS       sequence-123633-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kohinoor for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor/pRSETb
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..710)
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
     CDS             complement(join(720..734,735..743))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(747..779)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(783..800)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             820..842
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gggctttgtt agcagccgga tcaagcttcg aattcttact tggcctgcct cggcagccca
       61 gaatgcgctt cggcatgctc atgcagatta acattactgt aatctctgtc gtggctttta
      121 atctcaatgt ggtggaccac atcgtgatag tctggcaact ggacaacctt cttagcttta
      181 taagtagttt tggagtcaca tcggtagtgg ccacctcctt caagcgacag agcgtagttg
      241 ccatcgctct tcagcactcc atcacgcaca tacaatttct cagtggatgg ctcccatttc
      301 acagtcctct tctgcataac tggaccattg gcaggaaagt tcacaccatc aaatcgaatt
      361 tcatagatat aacagtcacc atccagggtt atgtcgtttg tcgcgataca aatgcccccg
      421 tcttcgtaaa tcatgcttcg ttcccaagag tagccctcag gaaacgactg cttgaaataa
      481 tctactatat tttctgggta tttggcgaat accctgttgc cgtaacagaa ggccattgtc
      541 aagatgtcat aggcgaaagg cagaggtccg ccttctttga ctttaaggtc catactctgt
      601 tttccctcga aaggcttccc aaggccaact ccttcaatcg cgaacgggtg tccatttaca
      661 gcgccttcca tacgcagctt gatcttcatg tctggtttaa tcacactcat ggtgggatcc
      721 ttatcgtcat cgtcgtacag atcccgaccc atttgctgtc caccagtcat gctagccata
      781 ccatgatgat gatgatgatg agaaccccgc atatgtatat ctccttctta aagttaaaca
      841 aaattatttc
//
