LOCUS       sequence-123637-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of cfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU3
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(128..148)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    557..590
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     599..615
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     599..615
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctatagaaat actcaagcta tgcatcaagc ttggtaccga gctcggatcc actagtaacg
       61 gccgccagtg tgctggaatt cgcccttttt gtatagttca tccatgccat gtgtaatccc
      121 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      181 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      241 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
      301 aattttgaag ttctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact
      361 taccgctttg atcccattct tttgtttgtc ggcagtgatg tatacgttgt gtgaaatata
      421 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
      481 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacctg aaaatttaaa
      541 tatgtatggt tagttagaag ttcctattct ctagaaagta taggaacttc gcggccgctc
      601 tagaactagt ggatcctcag cactgtcctg ctccttgtga tggtttacaa acgtaaaaag
      661 tctctttaat acctgttttt gcttcatatt gttcagcgac agcttgctgt acggcaggca
      721 ccagctcttc cgggatcagc gcgacgatac agccgccaaa tccgccgccg gtcatgcgta
      781 cgccaccttt gtcgccaatc acagctttga cgatttctac cagagtgtca atttgcggca
      841 cggtgatttc gaaatcatcg cgcatagagg catgagactc cgccatcaac tcgcccatac
      901 gtttcaggtc gccttgctcc agcgcgctgg cagcttcaac ggtgcgggcg ttttcagtca
      961 gtatatgacg cacgcgtttt gccacgatcg ggtccagttc atgcgcaaca gcgttgaact
     1021 cttcaatggt gacatcacgc agggctggct gctggaagaa acgcgcaccg gtttcgcact
     1081 gttcacgacg ggtgttgtat tcgctgccaa ccagggtacg tttgaagtta ctg
//
