LOCUS       sequence-123638-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PIF1 protein overproduction for purification from bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita 
            L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, 
            Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB
  TITLE     KELCH F-BOX protein positively influences Arabidopsis seed 
            germination by targeting PHYTOCHROME-INTERACTING FACTOR1.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 
            10.1073/pnas.1711919115. Epub 2018 Apr 9.
  PUBMED    29632208
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1711919115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-04-24- 24"; volume: "115"; issue: "17"; pages: 
            "E4120-E4129"
COMMENT     Sequence Label: pABD1275
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc ccgtggtatg gttgccgtga
       61 tcttcggatt ctttggagga ggagacgcga ctggaagacg gatagctcgt tgcctgattt
      121 tggaactgcg gcggctgggt cggatgcagg ccgcgaaact gttgatacgg atcggtgtag
      181 ccggccggat attgcggctg accgctggtc ggatcatact gttggttcgg gacaaaaacg
      241 cgcgacgggt ctgaagcatg caccggatac tgcggaccgc tacctgccat gtgggtctgg
      301 gtcggcagcg ggcgctgtgc cgccagcata ttcgggaacg gcataaaact cggcggcgga
      361 atcggctggt tcatgcccat acccatggcc atgtgcggca tatactgttg catacccggg
      421 tacatcatcg gcatcatgcc acaacccatc gacatcattt ggatctgcag ttgcagtgat
      481 ttcatgtatt caatggcttc gtccagcatg gacgctttat cacttttgtt gcagcgcggg
      541 atcagttcct gcagcgcttt catgcgttca ttaatacgat cgcgacgttt acgttcggac
      601 aggttatgca cttccgcagc gcgactacgt ttggtgctgg tcgtactaac gcgagcctgt
      661 ttcgtttctt cagaacggct ttcggtttcg tcggtcgtgg ttgcttcgcg ttctttgcgt
      721 ttacggtcat ccacattggt tttttccggt tcaatttccg attttgacac cacgctgctg
      781 ctggtgcccg tgatttcaat agccggtgcg gtcatggcga ccgctttacc cttacggtta
      841 tacgcgccac cgccagcaac tgcactagag tcggtaccat cggtgcgacg
//
