LOCUS       sequence-123639-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zyxin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Zyxin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccacc atggcggccc cccgcccgtc tcccgcgatc
      121 cccgtttcgg tctcggctcc ggctttttac gccccgcaga agaagttcgg ccctgtggtg
      181 gccccaaagc ccaaagtgaa tcccttccgg cccagggaca gcgagcctcc cccggcaccc
      241 ggggcccagc gcgcacagat gggccgggtg ggcgatattc ccccgccgcc cccggaagac
      301 tttcccctgc ctccacctcc ccttgctggg gatggcgacg atgcagaggg tgctctggga
      361 ggtgccttcc cgccgccccc tcccccgatc gaggaatcat ttccccctgc gcctctggag
      421 gaggagatct tcccttcccc gccgcctcct ccggaggagg agggagggcc tgaggccccc
      481 ataccgcccc caccacagcc cagggagaag gtgagcagta ttgatttgga gatcgactct
      541 ctgtcctcac tgctggatga catgaccaag aatgatcctt tcaaagcccg ggtgtcatct
      601 ggatatgtgc ccccaccagt ggccactcca ttcagttcca agtccagtac caagcctgca
      661 gccgggggca cagcacccct gcctccttgg aagtcccctt ccagctccca gcctctgccc
      721 caggttccgg ctccggctca gagccagaca cagttccatg ttcagcccca gccccagccc
      781 aagcctcagg tccaactcca tgtccagtcc cagacccagc
//
