LOCUS       sequence-123643-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of mChO (mCherry variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU4
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    529..562
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     571..587
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     571..587
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctagtagaat actcaagcta tgcatcaagc ttggtaccga gctcggatcc actagtaacg
       61 gccgccagtg tgctggaatt cgccctttta cttatacaat tcatccatgc cacctgtcga
      121 gtgccgccct tcagctcttt catattgctc aacgatagtg taatcctcgt tgtgggaggt
      181 gatatccagt ttaatattaa cattgtacgc tcctggcagc tgaactggtt ttttggcttt
      241 gtaagttgtc ttcacctgaa aatttaaatc agattagtta gttagtaaaa tcatgtttaa
      301 acttacctca gcatcgtaat gtcctccatc tttcaattta agacgttgtt taatctctcc
      361 cttaagagca ccgtcttcag ggtacattct ttcacttgaa gcttcccatc ccatagtctt
      421 cttctgcata acaggtccat ccgaggggaa attcgttcct cgaagtttga ctttataaat
      481 aaattctcca tcttggaggc tgaaaattta aatatgtatg gttagttaga agttcctatt
      541 ctctagaaag tataggaact tcgcggccgc tctagaacta gtggatcctc agcactgtcc
      601 tgctccttgt gatggtttac aaacgtaaaa agtctcttta atacctgttt ttgcttcata
      661 ttgttcagcg acagcttgct gtacggcagg caccagctct tccgggatca gcgcgacgat
      721 acagccgcca aatccgccgc cggtcatgcg tacgccacct ttgtcgccaa tcacagcttt
      781 gacgatttct accagagtgt caatttgcgg cacggtgatt tcgaaatcat cgcgcataga
      841 ggcatgagac tccgccatca actcgcccat acgtttcagg tcgccttgct ccagcgcgct
      901 ggcagcttca acggtgcggg cgttttcagt cagtatatga cgcacgcgtt ttgccacgat
      961 cgggtccagt tcatgcgcaa cagcgttgaa ctcttcaatg gtgacatcac gcagggctgg
     1021 ctgctggaag aaacgcgcac cggtttcgca ctgttcacga cgggtgttgt attcgctgcc
     1081 aaccagggta cgtttgaagt tactgttgat gatgacgacg
//
