LOCUS       sequence-123645-         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-mitochondria/pcDNA3
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..161
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             183..257
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 gagctctctg gctaactgag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccaccatgt ctgttctgac tcctctgctg ctccggggtc
      121 tcacaggttc cgcaagaaga ctccccgtgc ctagggccaa aattcattca ctgggggacc
      181 ccatgagcgt gctcacccca ctcctgctgc gggggctgac cggcagcgct aggcggctgc
      241 cagtccccag ggccaagatc cacagtctcg gcgatcccaa gcgcggccgc atcatgagtg
      301 tgattaaacc agacatgaag atcaagctgc gtatggaagg cgctgtaaat ggacacccgt
      361 tcgcgattga aggagttggc cttgggaagc ctttcgaggg aaaacagagt atggacctta
      421 aagtcaaaga aggcggacct ctgcctttcg cctatgacat cttgacaatg gccttctgtt
      481 acggcaacag ggtattcgcc aaatacccag aaaatatagt agattatttc aagcagtcgt
      541 ttcctgaggg
//
