LOCUS       sequence-123649-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golgi-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Golgi/pcDNA3
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagcttgc caccatgagg cttcgggagc cgctcctgag cggcagcgcc gcgatgccag
      121 gcgcgtccct acagcgggcc tgccgcctgc tcgtggccgt ctgcgctctg caccttggcg
      181 tcaccctcgt ttactacctg gctggccgcg acctgagccg cctgccccaa ctggtcggag
      241 tctccacacc gctgcagggc ggctcgaaca gtgccgccgc catcgggcag tcctccgggg
      301 agctccggac cggaggggcc ggatccatga gtgtgattaa accagacatg aagatcaagc
      361 tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt ggccttggga
      421 agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga cctctgcctt
      481 tcgcctatga catcttgaca atggccttct gttacggcaa cagggtattc gccaaatacc
      541 cagaaaatat agtagattat ttcaagcagt cgtttcctga gggctactct tgggaacgaa
      601 gcatgattta cgaagacggg ggcatttgta tcgcgacaaa cgacataacc ctggatggtg
      661 actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat ggtccagtta
      721 tgcagaagag gactgtg
//
