LOCUS       sequence-123654-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: Venus in frame 
            w/ FRT-galK-FRT; no introns; N-terminal fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU6
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(70..72,73..75,76..786)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(115..136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     723..744
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(789..822)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        826..873
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ggcgattggg ccctctgatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgccctta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      181 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
      241 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
      301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      361 accatcttct tcaaggacga cggcaattac aagacccgcg ccgaggtgaa gttcgagggc
      421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
      541 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
      601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      661 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      781 tacaagggga agttcctata ctttctagag aataggaact tccctgttga caattaatca
      841 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca
      901 gatcatgagt ctgaaagaaa aaacacaatc tctgtttgcc aacgcatttg gctaccctgc
      961 cactcacacc attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa
     1021 cgacggtttc gttctgccct gcgcgattga ttatcaaa
//
