LOCUS       sequence-123655-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: Venus in frame 
            w/ FRT-galK-FRT; no introns; N-terminal fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU6
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..120
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     129..145
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     129..145
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
       61 ccgccagtgt gctggaattc gcccttgaag ttcctattct ctagaaagta taggaacttc
      121 gcggccgctc tagaactagt ggatcctcag cactgtcctg ctccttgtga tggtttacaa
      181 acgtaaaaag tctctttaat acctgttttt gcttcatatt gttcagcgac agcttgctgt
      241 acggcaggca ccagctcttc cgggatcagc gcgacgatac agccgccaaa tccgccgccg
      301 gtcatgcgta cgccaccttt gtcgccaatc acagctttga cgatttctac cagagtgtca
      361 atttgcggca cggtgatttc gaaatcatcg cgcatagagg catgagactc cgccatcaac
      421 tcgcccatac gtttcaggtc gccttgctcc agcgcgctgg cagcttcaac ggtgcgggcg
      481 ttttcagtca gtatatgacg cacgcgtttt gccacgatcg ggtccagttc atgcgcaaca
      541 gcgttgaact cttcaatggt gacatcacgc agggctggct gctggaagaa acgcgcaccg
      601 gtttcgcact gttcacgacg ggtgttgtat tcgctgccaa ccagggtacg tttgaagtta
      661 ctgttgatga tgacgacagc cacacctttg ggcatggaaa ctgctttggt ccccagtgag
      721 cggcaatcga tcagcaaggc atgatctttc ttgccgagcg cggaaattag ctgatccatg
      781 atcccgcagt tacagcctac aaactggttt tctgcttcct gaccgttaag cgcgatttgt
      841 gcgccgtcca gcggcagatg ataaagctgc tgcaatacgg ttccgaccgc gacttccagt
      901 gaagcggaag aacttaaccc ggcaccctgc ggcacattgc cgctgatcac catgtccacg
      961 ccgccgaagc tgttgttacg cagttgcaga tgtttcacca cgccacgaac gtagttagcc
     1021 cattgatagt tttcatgtgc gacaatgggc gcatcgaggg aaaa
//
