LOCUS       sequence-123664-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fibrilarin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Fibrilarin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..762
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSELPRQ
                     AK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccccacc atgagtgtga ttaaaccaga catgaagatc
      121 aagctgcgta tggaaggcgc tgtaaatgga cacccgttcg cgattgaagg agttggcctt
      181 gggaagcctt tcgagggaaa acagagtatg gaccttaaag tcaaagaagg cggacctctg
      241 cctttcgcct atgacatctt gacaatggcc ttctgttacg gcaacagggt attcgccaaa
      301 tacccagaaa atatagtaga ttatttcaag cagtcgtttc ctgagggcta ctcttgggaa
      361 cgaagcatga tttacgaaga cgggggcatt tgtatcgcga caaacgacat aaccctggat
      421 ggtgactgtt atatctatga aattcgattt gatggtgtga actttcctgc caatggtcca
      481 gttatgcaga agaggactgt gaaatgggag ccatccactg agaaattgta tgtgcgtgat
      541 ggagtgctga agagcgatgg caactacgct ctgtcgcttg aaggaggtgg ccactaccga
      601 tgtgactcca aaactactta taaagctaag aaggttgtcc agttgccaga ctatcacgat
      661 gtggtccacc acattgagat taaaagccac gacagagatt acagtaatgt taatctgcat
      721 gagcatgccg aagcgcattc tgagctgccg aggcaggcca aggaattcat gaagccagga
      781 ttcagt
//
