LOCUS       sequence-123668-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID); HA 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Roux KJ, Kim DI, Raida M, Burke B
  TITLE     A promiscuous biotin ligase fusion protein identifies proximal and 
            interacting proteins in mammalian cells.
  JOURNAL   J Cell Biol. 2012 Mar 12.
  PUBMED    22412018
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2012 Mar 12."
COMMENT     Sequence Label: pcDNA3.1 MCS-BirA(R118G)-HA
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgct taaggcctgt taaccggtcg tacgtctccg
      121 gattcgaatt cggatccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca
      181 acggcgagtt ccactctggc gagcagctgg gagagaccct gggaatgagc agagccgcca
      241 tcaacaagca catccagaca ctgagagact ggggagtgga cgtgttcacc gtgcctggca
      301 agggctacag cctgcctgag cctatccagc tgctgaacgc caagcagatc ctgggacagc
      361 tggatggcgg aagcgtggcc gtgctgcctg tgatcgactc caccaatcag tacctgctgg
      421 acagaatcgg agagctgaag tccggcgacg cctgcatcgc cgagtaccag caggctggca
      481 gaggaggcag aggacggaag tggttcagcc cattcggagc caacctgtac ctgtccatgt
      541 tctggagact ggagcaggga cctgctgctg ccatcggact gagtctggtg atcggaatcg
      601 tgatggccga ggtgctgaga aagctgggag ccgacaaggt gagagtgaag tggcctaatg
      661 acctgtacct ccaggaccgc aagctggctg gcatcctggt ggagctgaca ggcaagacag
      721 gcgatgccgc tcagatcgtg atcggagccg gaatcaacat ggccatgaga agagtggagg
      781 agagcgtggt gaaccagggc tggatcaccc tgcaggaggc tggcatcaac ctggaccg
//
