LOCUS       sequence-123678-        1708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-binding protein MS2 with eDHFR fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Carrocci TJ, Hoskins AA
  TITLE     Imaging of RNAs in live cells with spectrally diverse small molecule
            fluorophores.
  JOURNAL   Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 
            Nov 1.
  PUBMED    24187681
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3an01550e"; journalName: "Analyst"; date: "2014-01-7- 7"; 
            volume: "139"; issue: "1"; pages: "44-7"
COMMENT     Sequence Label: MS2-ecDHFR
FEATURES             Location/Qualifiers
     source          1..1708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             134..145
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             539..1018
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     promoter        1024..1429
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac accaagaact
       61 tagtttcgac ggatccacca tgccaaaaaa gaaaagaaaa gttggctacc cctacgacgt
      121 gcccgactac gccatcgaag gccgccatat gctagccgtt aaaatggctt ctaactttac
      181 tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt
      241 cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac
      301 ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc
      361 taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa
      421 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
      481 tccctcagca atcgcagcaa actccggcat ctacgcggcc gctggttctg gttctggtat
      541 gatcagtctg attgcggcgt tagcggtaga tcgcgttatc ggcatggaaa acgccatgcc
      601 gtggaacctg cctgccgatc tcgcctggtt taaacgcaac accttaaata aacccgtgat
      661 tatgggccgc catacctggg aatcaatcgg tcgtccgttg ccaggacgca aaaatattat
      721 cctcagcagt caaccgggta cggacgatcg cgtaacgtgg gtgaagtcgg tggatgaagc
      781 catcgcggcg tgtggtgacg taccagaaat catggtgatt ggcggcggtc gcgtttatga
      841 acagttcttg ccaaaagcgc aaaaactgta tctgacgcat atcgacgcag aagtggaagg
      901 cgacacccat ttcccggatt acgagccgga tgactgggaa tcggtattca gcgaattcca
      961 cgatgctgat gcgcagaact ctcacagcta ttgctttgag attctggagc ggcggtaagt
     1021 taaaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca
     1081 ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc
     1141 gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact
     1201 gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag
     1261 agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat attatttaag
     1321 gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc
     1381 ttttacattt cagcaatata tatatatatt tcaaggatat accattctaa tgtctgcccc
     1441 taagaagatc gtcgttttgc caggtgacca cgttggtcaa gaaatcacag ccgaagccat
     1501 taaggttctt aaagctattt ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca
     1561 tttaattggt ggtgctgcta tcgatgctac aggtgttcca cttccagatg aggcgctgga
     1621 agcctccaag aaggctgatg ccgttttgtt aggtgctgtg ggtggtccta aatggggtac
     1681 cggtagtgta gaccgaacaa ggtacaat
//
