LOCUS       sequence-12368-e        1979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP2
FEATURES             Location/Qualifiers
     source          1..1979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..82
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1923..1940
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gtcatcatca
       61 tcatcatcac attgagggac gtctgaagct gttggacaat tgggactctg ttacgtctac
      121 cttcagtaaa cttcgcgaac aactgggccc cgtgacgcag gaattctggg acaacctgga
      181 aaaagaaacc gagggactgc gtcaggaaat gtccaaagat ttagaagagg tgaaggccaa
      241 ggttcagcca tatctcgatg actttcagaa aaaatggcag gaagagatgg aattatatcg
      301 tcaaaaggtg gaaccgctgc gtgcggaact gcaagagggg gcacgccaaa aactccatga
      361 gctccaagag aagctcagcc cattaggcga agaaatgcgc gatcgcgccc gtgcacatgt
      421 tgatgcactc cggactcatt tggcgccgta ttcggatgaa cttcgccagc gtttggccgc
      481 acgtctcgag gcgctgaaag aaaacggggg tgcccgcttg gctgagtacc acgcgaaagc
      541 gacagaacac ctgagcacct tgagcgaaaa agcgaaaccg gcgctggaag atctacgcca
      601 gggcttattg cctgttcttg agagctttaa agtcagtttt ctgtcagctc tggaagaata
      661 tactaaaaag ctgaataccc agggtaccct gaagctgttg gacaattggg actctgttac
      721 gtctaccttc agtaaacttc gcgaacaact gggccccgtg acgcaggaat tctgggacaa
      781 cctggaaaaa gaaaccgagg gactgcgtca ggaaatgtcc aaagatttag aagaggtgaa
      841 ggccaaggtt cagccatatc tcgatgactt tcagaaaaaa tggcaggaag agatggaatt
      901 atatcgtcaa aaggtggaac cgctgcgtgc ggaactgcaa gagggggcac gccaaaaact
      961 ccatgagctc caagagaagc tcagcccatt aggcgaagaa atgcgcgatc gcgcccgtgc
     1021 acatgttgat gcactccgga ctcatttggc gccgtattcg gatgaacttc gccagcgttt
     1081 ggccgcacgt ctcgaggcgc tgaaagaaaa cgggggtgcc cgcttggctg agtaccacgc
     1141 gaaagcgaca gaacacctga gcaccttgag cgaaaaagcg aaaccggcgc tggaagatct
     1201 acgccagggc ttattgcctg ttcttgagag ctttaaagtc agttttctgt cagctctgga
     1261 agaatatact aaaaagctga atacccaggg taccctgaag ctgttggaca attgggactc
     1321 tgttacgtct accttcagta aacttcgcga acaactgggc cccgtgacgc aggaattctg
     1381 ggacaacctg gaaaaagaaa ccgagggact gcgtcaggaa atgtccaaag atttagaaga
     1441 ggtgaaggcc aaggttcagc catatctcga tgactttcag aaaaaatggc aggaagagat
     1501 ggaattatat cgtcaaaagg tggaaccgct gcgtgcggaa ctgcaagagg gggcacgcca
     1561 aaaactccat gagctccaag agaagctcag cccattaggc gaagaaatgc gcgatcgcgc
     1621 ccgtgcacat gttgatgcac tccggactca tttggcgccg tattcggatg aacttcgcca
     1681 gcgtttggcc gcacgtctcg aggcgctgaa agaaaacggg ggtgcccgct tggctgagta
     1741 ccacgcgaaa gcgacagaac acctgagcac cttgagcgaa aaagcgaaac cggcgctgga
     1801 agatctacgc cagggcttat tgcctgttct tgagagcttt aaagtcagtt ttctgtcagc
     1861 tctggaagaa tatactaaaa agctgaatac ccagtaataa gcttgcctag gccgcactcg
     1921 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag aagctgagt
//
