LOCUS       sequence-123684-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2ndmChO intron; use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU18
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             288..308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(744..777)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        781..828
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gggcgattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 aattcgccct tgccatgttg ttaccttgta ttaaaacaat gtttcaagtt gaatttgttc
      121 ttgttgttga attctattca catggtcttt acccatgctt catatcttat tgttttctta
      181 ccctggtcac tttgtggcat taatactttc tttcagcgtt ctatcaaacg tttgttctct
      241 ttggagctaa ggcaaaccgc gcaacttacg attgaagaat gaccgctcca aagaagaaac
      301 gcaaagtacc ggtagaaaaa atggtctcaa agggtgaaga agataacatg gcaattatta
      361 aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat gagtttgaaa
      421 ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa
      481 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta actaaaggcg
      541 gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga agtaaagctt
      601 atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg gagggtttta
      661 agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg caagactcaa
      721 ggtaagttta aacagttcgg tacgaagttc ctatactttc tagagaatag gaacttccct
      781 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
      841 taaacccagg aggcagatca tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc
      901 atttggctac cctgccactc acaccattca ggcgcctggc cgcgtgaatt tgattggtga
      961 acacaccgac tacaacgacg gtttcgttct gccctgcgcg attgattatc aaaccgtgat
     1021 cagttgtgca ccacgcgatg accgtaaagt tcgcgtgatg gcagccga
//
