LOCUS       sequence-123685-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2ndmChO intron; use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU18
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    531..564
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     573..589
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     573..589
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tgactagtag aatactcagc tatgcatcaa gcttggtacc gagctcggat ccactagtaa
       61 cggccgccag tgtgctggaa ttcgcccttt tacttataca attcatccat gccacctgtc
      121 gagtgccgcc cttcagctct ttcatattgc tcaacgatag tgtaatcctc gttgtgggag
      181 gtgatatcca gtttaatatt aacattgtac gctcctggca gctgaactgg ttttttggct
      241 ttgtaagttg tcttcacctg aaaatttaaa tcagattagt tagttagtaa aatcatgttt
      301 aaacttacct cagcatcgta atgtcctcca tctttcaatt taagacgttg tttaatctct
      361 cccttaagag caccgtcttc agggtacatt ctttcacttg aagcttccca tcccatagtc
      421 ttcttctgca taacaggtcc atccgagggg aaattcgttc ctcgaagttt gactttataa
      481 ataaattctc catcttggag gctgaaaatt taaatatgta tggttagtta gaagttccta
      541 ttctctagaa agtataggaa cttcgcggcc gctctagaac tagtggatcc tcagcactgt
      601 cctgctcctt gtgatggttt acaaacgtaa aaagtctctt taatacctgt ttttgcttca
      661 tattgttcag cgacagcttg ctgtacggca ggcaccagct cttccgggat cagcgcgacg
      721 atacagccgc caaatccgcc gccggtcatg cgtacgccac ctttgtcgcc aatcacagct
      781 ttgacgattt ctaccagagt gtcaatttgc ggcacggtga tttcgaaatc atcgcgcata
      841 gaggcatgag actccgccat caactcgccc atacgtttca ggtcgccttg ctccagcgcg
      901 ctggcagctt caacggtgcg ggcgttttca gtcagtatat gacgcacgcg ttttgccacg
      961 atcgggtcca gttcatgcgc aacagcgttg aactcttcaa tggtgacatc acgcagggct
     1021 ggctgctgga agaaacgcgc accggtttcg cactgttcac gacgggtgtt gtattcgc
//
