LOCUS       sequence-123686-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aldehyde dehydrogenase from Rattus norvegicus, codon 
            optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     Siegel Lab ALDH Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: rnALDH
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    92..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..153
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
       61 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      121 tagaaataat tttgtttaac tttaagaagg agatatacat atgagcagca ttagcgatac
      181 cgttaaacgt gcccgtgaag catttaatag cggtaaaacc cgtagcctgc aatttcgtat
      241 tcagcagctg gaagcactgc aacgtatgat taatgaaaac ctgaaaagca ttagtggtgc
      301 actggcaagc gatctgggta aaaatgaatg gaccagctat tatgaagaag ttgcacacgt
      361 tctggaagaa ctggatacca ccattaaaga actgccggat tgggcagaag atgaacctgt
      421 tgcaaaaacc cgtcagaccc agcaggatga tctgtatatt catagcgaac cgctgggtgt
      481 tgttctggtt attggtgcat ggaattatcc gtttaatctg accattcagc cgatggttgg
      541 tgcagttgca gcaggtaatg cagttattct gaaaccgagc gaagttagcg gtcacatggc
      601 cgatctgctg gcaaccctga ttccgcagta tatggatcag aatctgtatc tggttgttaa
      661 aggtggtgtg ccggaaacca ccgaactgct gaaagaacgt tttgatcata ttatgtatac
      721 cggcagcacc gcagttggta aaattgttat ggcagcagca gcaaaacatc tgactccggt
      781 taccctggaa ctgggtggta aaagcccgtg ttatgttgat aaagattgcg atctggatgt
      841 tgcctgtcgt cgtattgcat ggggcaaatt tatgaatagc ggtcagacct gtgttgcacc
      901 ggattatatt ctgtgtgatc cgagcattca gaaccagatt gttgagaaac tgaaaaaa
//
