LOCUS       sequence-123689-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, 
            codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_WT
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    92..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..153
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
       61 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      121 tagaaataat tttgtttaac tttaagaagg agatatacat atgaaactga acaaacagaa
      181 tctgacccag ctggcaccgg aagttaaact gcctgcatat accctggcag atacccgtca
      241 gggtattgca catattggtg ttggtggttt tcatcgtgca catcaggcat attataccga
      301 tgcactgatg aatacaggtg aaggtctgga ttggagcatt tgcggtgttg gtctgcgtag
      361 cgaagatcgt aaagcacgtg atgatctggc aggtcaggat tacctgttta ccctgtatga
      421 actgggcgat accgatgata ccgaagttcg tgttattggt agcattagcg atatgctgct
      481 ggcagaagat agcgcacagg cactgattga taaactggca agtccggaaa ttcgtattgt
      541 tagcctgacc attaccgaag gtggttattg tattgatgat agcaacggtg aatttatggc
      601 acatctgccg cagattcagc acgatctggc acatccgagc agcccgaaaa ccgtttttgg
      661 ttttatttgt gcagcactga cacagcgtcg tgcagcaggt attccggcat ttaccgttat
      721 gagctgtgat aacctgccgc ataatggtgc agttacccgt aaagccctgc tggcatttgc
      781 agccctgcat aatgcagaac tgcatgattg gattaaagcc catgttagct ttccgaatgc
      841 aatggttgat cgtattaccc cgatgaccag caccgcacat cgtctgcaac tgcatgacga
      901 acatggtatt gatgatgcat ggcctgttgt ttgtgaaccg tttgttcagt gggttctgga
      961 agataaattt gttaatggtc gtccggcatg ggaaaaagtt ggtgttcagt ttaccgatga
     1021 tgttaccccg tatgaagaaa tgaaaattgg tctgctgaat ggtagccatc tggcactgac
     1081 ctatctgggt tttctgaaag gttatcgttt tgtgcatgaa acc
//
