LOCUS       sequence-123690-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, 
            codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_WT
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga ggctaccaac aggttttttc
       61 agcagatgtt tcagggtggt ggtaacacca ttatcacgca ggctaccata acaacgttca
      121 aatgcggcaa caaattccgg actattcgga attgcggtgc caaaaatttc ttcaactgcc
      181 agcagacgct ggctaatcag tgcatcatct gaaaccagac cctgacaaaa ttctgcacgc
      241 ggatcaggaa tggtatagct cacaccattt tcatcaacgc ctttcagata cagtgcccat
      301 gctgcaacaa ccagtgctgc acgttcggtt tcacgaccat ctgcaatcag acgattaatt
      361 gtcggaacgg taaatttagg aaatttgctg ctaccatcac tacaaacacg ttccagctga
      421 tctgcaattg cctgattgct aaaacgatca accagggtct gtttataatc ggtcagatca
      481 atacccggaa ccggagccag attcggggtc acatccagat ccatatatgc acgcatatag
      541 gcaacaaaca gcggatcgtt catggtttca tgcacaaaac gataaccttt cagaaaaccc
      601 agataggtca gtgccagatg gctaccattc agcagaccaa ttttcatttc ttcatacggg
      661 gtaacatcat cggtaaactg aacaccaact ttttcccatg ccggacgacc attaaca
//
