LOCUS       sequence-123695-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +0
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..23)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cacaccttgc gcttcttctt ggggtcggcg cggctgtcgc cgcccagctg gctcaggtcg
       61 atgcgggtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctcc
      121 ttggtgctgg tgtagcgctt gcggtcgatg gtggtgtcga agtacttgaa ggcggcgggg
      181 gcgcccaggt tggtcagggt gaacaggtgg atgatgttct cggcctgctc gcggatgggc
      241 ttgtcgcggt gcttgttgta ggcgctcagc accttgtcca ggttggcgtc ggccaggatc
      301 acgcgcttgc tgaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgc
      361 tccacgaaca gctgcttctg ctcgttgtcc tcggggctgc ccttcagctt ctcgtagtgg
      421 ctggccaggt acaggaagtt cacgtacttg ctgggcaggg ccagctcgtt gcccttctgc
      481 agctcgccgg cgctggccag catgcgcttg cggccgttct ccagctcgaa caggctgtac
      541 ttgggcagct tgatgatcag gtccttcttc acctccttgt agcccttggc ctccaggaag
      601 tcgatggggt tcttctcgaa gctgctgcgc tccatgatgg tgatgcccag cagctccttc
      661 acgctcttca gcttcttgct cttgcccttc tccaccttgg ccaccaccag cacgctgtag
      721 gccacggtgg ggctgtcgaa gccgccgtac ttcttggggt cccagtcctt cttgcgggcg
      781 atcagcttg
//
