LOCUS       sequence-123700-        7375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral pBabe puro vector encoding N-terminally FLAG tagged 
            mutant mouse PERK deleted from amino acids M1 to Y502. The remaining
            amino acids are S503 to N1114. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_MmPERK_S503_N1114_pBabePuro
FEATURES             Location/Qualifiers
     source          1..7375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    523..722
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             923..1339
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1269..1291
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1307..1323
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1396..1440
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             1441..1464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3686..3706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3691..4020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3871..4006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4030..4629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4030..4049)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4526..4546
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4850..5319
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5449..5466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5620..6208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5700..5719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6368..7159,7160..7228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6991..7010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7229..7333)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(7364..7375,1..458)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
       61 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      121 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
      181 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
      241 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
      301 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
      361 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
      421 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
      481 ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc cagcaactta
      541 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
      601 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
      661 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
      721 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
      781 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
      841 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
      901 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
      961 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1021 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1081 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1141 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1201 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1261 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1321 cactccttct ctaggcgccg gccggatccc agtgtggtgg tacctcgttt agtgaaccgt
     1381 cagaattaat tcaccatgtc tgcacttctg atcctagctc ttgttggagc tgcagttgct
     1441 gactacaaag acgatgacga caagcttgcg gccgccagca agaacatccg caagaaggac
     1501 cctatcctcc tgctgcactg gtggaaggag atattcggga cgatcctgct ttgcatcgta
     1561 gccacgacct tcatcgtgcg caggcttttc catcctcagc cccacaggca gcggaaggag
     1621 tctgaaactc agtgccagac tgaaagtaaa tacgactccg tgagtgccga tgtcagtgac
     1681 aacagctgga atgacatgaa gtactcagga tacgtatccc gatatctaac agattttgag
     1741 ccaattcagt gcatgggtcg tggtggcttt ggcgttgtct ttgaagctaa aaacaaagta
     1801 gatgactgca attacgctat caagaggatc cggctcccca acagggagtt ggcacgggag
     1861 aaggtaatgc gggaagttaa agccttggct aagctggaac acccaggcat tgtgaggtat
     1921 ttcaacgcct ggctggaaac cccaccagag aagtggcaag aagagatgga tgagatctgg
     1981 ctcaaagacg aaagcacaga ctggccgctc agctccccta gcccgatgga tgccccatct
     2041 gttaagatcc gaaggatgga tcctttctct acaaaagagc agatcgaagt catagctcct
     2101 tctcctgaaa gaagtcggtc tttctcggtg ggcatttcct gtggccagac aagctcatcg
     2161 gagagccagt tctctcccct ggagttctca gggacagact gcggagacaa cagtgactca
     2221 gcggacgcag cctacaacct ccaggacagt tgcctgacgg actgcgagga cgtggaagat
     2281 ggcaccgtgg acggcaatga cgagggacac tcctttgaac tttgtccgtc cgaagcttct
     2341 ccctataccc ggtctaggga aggaacgtcc tcctccatag tgtttgagga ctctggctgc
     2401 ggcaacgcgt ccagtaagga ggagcccaga gggaaccggc tgcatgatgg caaccattat
     2461 gttaataagc taactgatct caagtgctcc agcagcaggt cttcttcaga agccaccacc
     2521 ttgtctacct cccctaccag gccaaccact ctaagcttgg atttcaccaa gaacactgtg
     2581 ggccagctcc agcccagctc ccccaaggtg tatctgtaca ttcagatgca gctgtgcagg
     2641 aaggagaacc tcaaagactg gatgaaccgg cgctgcagct tggaggaccg ggagcacggc
     2701 gtgtgcctgc acatcttcct gcagatcgca gaggcagtgg agttcctgca cagcaaggga
     2761 ctcatgcaca gggacctcaa gccttccaac atattcttca caatggatga tgtggtcaag
     2821 gttggggact ttggactggt gactgctatg gaccaagatg aagaagagca gactgtactg
     2881 actccaatgc cagcctatgc tacgcacacg ggacaagtag ggaccaagct atacatgagc
     2941 ccagagcaga ttcatggaaa caactactcc cataaagtgg acatcttctc tttaggcttg
     3001 attctgtttg aactcctcta cccattcagc acccagatgg aacgagtccg gattttaact
     3061 gatgtcagaa atctcaagtt tcctctactg ttcactcaga aatatcccca agagcatatg
     3121 atggttcaag acatgctctc tccatccccc acggagcggc ctgaagccac agacatcatt
     3181 gaaaatgcca tatttgagaa cttggagttt cccgggaaaa cggttctgag acagcggtcc
     3241 cgctccatga gttcatctgg aacaaaacat tccagacagc ccagctgctc gtacagccca
     3301 ctgcctggca actagccctc agctgccctc gaaggtggca gagcaggcac cctgaggaac
     3361 atggctctcc acagcggtgg actcagattt tatgctttga tcagttggac tcgggaccaa
     3421 tttttctaag tcagactgga tcacgggcct aacccagttt gatcttaact gaacttcaag
     3481 gaaagggctg tgtaaaggac acatgaactt gttgcttgtc ggtgtcccaa gactagctgg
     3541 tcagcttaga accttcactt ttcaccaggc ggtagaagag atcctcaaat ggtctgaact
     3601 ggaaatgtct ttaaagcaca aaagtgtaaa agaccctctc acatgggaac tacatgttct
     3661 agcttatcga taccgtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4021 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4081 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4141 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4201 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     4261 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     4321 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     4381 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     4441 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     4501 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     4561 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     4621 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     4681 gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     4741 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     4801 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     4861 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     4921 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     4981 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     5041 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     5101 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     5161 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     5221 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     5281 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     5341 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6301 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     6361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7321 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggc
//
