LOCUS       sequence-123702-        5598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: mCherry-UtrCH
FEATURES             Location/Qualifiers
     source          1..5598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..798
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(236..254)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     504..523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1650..1784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1674..1693)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1728..1747
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1897..1917)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1897..1915)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1936..1952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1936..1952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1949..1971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1960..1976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1984..1990,1991..2008,2009..2014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2029..2050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2167..2184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2338..2926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2418..2437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3097..3888,3889..3957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3720..3739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3958..4062)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4088..4543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4437..4456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4614..5598
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccgccacc atggtgagca agggcgagga ggataacatg
      121 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      241 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      301 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      361 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      421 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      481 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      541 tcctccgaga gaatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      601 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      661 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      721 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      781 atggacgagc tgtacaagtc cggaaccatg gccaagtatg gagaacatga agccagtcct
      841 gacaatgggc agaacgaatt cagtgatatc attaagtcca gatctgatga acacaatgac
      901 gtacagaaga aaacctttac caaatggata aatgctcgat tttcaaagag tgggaaacca
      961 cccatcaatg atatgttcac agacctcaaa gatggaagga agctattgga tcttctagaa
     1021 ggcctcacag gaacatcact gccaaaggaa cgtggttcca caagggtaca tgccttaaat
     1081 aacgtcaaca gagtgctgca ggttttacat cagaacaatg tggaattagt gaatataggg
     1141 ggaaccgaca ttgtggatgg aaatcacaaa ctgactttgg ggttactttg gagcatcatt
     1201 ttgcactggc aggtgaaaga tgtcatgaag gatgtcatgt cggacctgca gcagacgaac
     1261 agtgagaaga tcctgctcag ctgggtgcgt cagaccacca ggccctacag ccaagtcaac
     1321 gtcctcaact tcaccaccag ctggacagat ggactcgcct ttaatgctgt cctccaccga
     1381 cataaacctg atctcttcag ctgggataaa gttgtcaaaa tgtcaccaat tgagagactt
     1441 gaacatgcct tcagcaaggc tcaaacttat ttgggaattg aaaagctgtt agatcctgaa
     1501 gatgttgccg ttcggcttcc tgacaagaaa tccataatta tgtatttaac atctttgttt
     1561 gaggtgctac ctcagcaagt caccatagac taactcgagc tcaagcttcg aattctgcag
     1621 tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt
     1681 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1741 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1801 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg
     1861 cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     1921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1981 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2161 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2221 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2281 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2341 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2401 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2461 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2521 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2581 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2641 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2701 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2761 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2821 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2881 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2941 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3001 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3061 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3181 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3241 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3541 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3661 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3721 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3961 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4021 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4081 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4141 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4201 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4261 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4321 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4381 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4441 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4501 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagtcgacc
     4621 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
     4681 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
     4741 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
     4801 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
     4861 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
     4921 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
     4981 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
     5041 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
     5101 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
     5161 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
     5221 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
     5281 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
     5341 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
     5401 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
     5461 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
     5521 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
     5581 aatgctcgtt tagggaac
//
