LOCUS       sequence-123718-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal MBP tagged human MEK1 gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: MBP-MEK1
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..29
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             30..50
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataacaacaa cctcgggatc gagggaaggg aaaatctgta ttttcaaggt catatgccga
       61 agaagaaacc gaccccgatc cagctgaacc cggctccgga cggttctgct gttaacggca
      121 cctcttctgc tgaaaccaac ctggaagctc tgcaaaagaa actggaagaa ctggaactgg
      181 acgaacagca gcgtaaacgt ctggaagcgt tcctgaccca gaaacagaaa gttggtgaac
      241 tgaaagacga cgacttcgaa aaaatctctg aactgggtgc tggtaacggt ggtgttgttt
      301 tcaaagtttc tcacaaaccg tccggtctgg ttatggctcg taaactgatc cacctggaaa
      361 tcaaaccggc tatccgtaac cagatcatcc gtgaactgca agttctgcac gaatgcaact
      421 ctccgtacat cgttggtttc tacggtgctt tctactctga cggtgaaatc tctatctgca
      481 tggaacacat ggacggtggt tctctggacc aggttctgaa aaaagctggt cgtatcccgg
      541 aacagatcct gggtaaagtt tctatcgctg ttatcaaagg tctgacctac ctgcgtgaaa
      601 aacacaaaat catgcaccgt gacgttaaac cgtctaacat cctggttaac tctcgtggtg
      661 aaatcaaact gtgcgacttc
//
