LOCUS       sequence-123721-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal MBP tagged human MEK1 gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: MBP-MEK1
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..71)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat caagcttgtc gacggagctc gaattcggat ccggcgcgcc ttagtgatgg
       61 tggtgatgat gcgcagctgc ggctgccaca ccagcagcgt gggtcggggt agacggctgg
      121 ttcagaccga tggtagagca cagccaacca gcgaagtcaa cttcttcagc gtcagaacgt
      181 ttgatgaaag cgtgaaccat cagctgtttc aggtcagcac gttcagccgg gtttttgatc
      241 aggcatttgt taacgaagtc ctggaactcc agagagaaaa caccagacgg cagtttcgga
      301 ggcggttcgt taacgatgta gtccagcagt tcgaagatag ccatcggcgg acgagagtcc
      361 ataccgtaag aagacagcgg acgacccgga gtacgcggac gcggcggggt ttcagcagcg
      421 tcaccttcaa cctggcaacc gaacatcagt tccagttctt tagcgtccgg cggcgggatc
      481 gggtaacgac caacagccat ttcaaccaga gacagaccca tagaccagat gtcagactga
      541 acagagtagt gggtgccttg cagacgttcc ggagacatgt aagaacgggt gccaacgaaa
      601 gagttagcca tagagtcgat cagctgacca gaaacaccga agtcgcacag tttgatttca
      661 ccacgagagt taaccaggat gttagacggt ttaacgtcac ggtgcatgat tttgtgtttt
      721 tcacgcaggt aggtcagacc tttgataaca gcgatagaaa ctttacccag gatctgttcc
      781 gggatacgac cagctttttt cagaacctgg tccagagaac caccgtccat gtgttccatg
      841 cagatagaga tttcaccgtc agagtagaaa gcaccgtaga aaccaacgat gtacggagag
      901 ttgcattcgt gcagaacttg cagttcacgg atgatctggt tacggatagc
//
