LOCUS       sequence-123736-        6125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of P. falciparum dihydropteroate synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Stratton CF, Namanja-Magliano HA, Cameron SA, Schramm VL
  TITLE     Binding Isotope Effects for para-Aminobenzoic Acid with 
            Dihydropteroate Synthase from Staphylococcus aureus and Plasmodium 
            falciparum.
  JOURNAL   ACS Chem Biol. 2015 Oct 16;10(10):2182-6. doi: 
            10.1021/acschembio.5b00490. Epub 2015 Aug 27.
  PUBMED    26288086
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00490"; journalName: "ACS Chem Biol"; date: 
            "2015-10-16- 16"; volume: "10"; issue: "10"; pages: "2182-6"
COMMENT     Sequence Label: P. falciparum  DHPS
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    94..113
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             2287..2304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2771..2788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2771..2787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2886..2903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3057..3645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3137..3156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3731..4813)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4775..4794
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(join(4896..5687,5688..5756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5519..5538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5757..5848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5890..5909
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5915..5934
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttaggagg taaaacatat ggagactatt
      181 caagaactga ttctgagcga agagaacaaa acgaatattg cggtgctgaa tttgggcacg
      241 aatgaccgcc gtaacgcggt tctgatcctg gaaacggccc tgcacctggt ggagaagtac
      301 ctgggtaaga ttatcaacac cagctacctg tatgagactg tcccggagta tattgtcttg
      361 gacaagaaag agagctgtga aaagatcaat aaagactgcc gcatctacga tgtcaactat
      421 atcaatgagc tgatgcaaaa tctggaagaa agcaagtatg aagagaataa agagctgatt
      481 gataagtgcg aagaatacga aacctttctg aagaatggca aggttgataa tagcatcctg
      541 aaagaggtca acgtcgagaa ctacttgctg gagtgcaata acatcatcgt caaaaacgat
      601 gaaatcatga aaaacaacct gagcaagtac aaagacaaat actatacgtc ttacttttac
      661 aatctgaccg tcgtggtgaa aaccttcgtt aatgatccgc tgagcatgct ggtggtcatc
      721 aaatacatcg aagagctgat gaaacgtgaa aatgttaaag agaaagagaa gtttgagaac
      781 cgtattatcg acatcgacat tctgtttttc aacgatttca cgattttcat gaagaacatt
      841 aaactggaga aaaacatgat ttacaagatt ctgtctaaat acatccacct ggaacgtgac
      901 atcaagaacg gtaacgacaa tatgtcgaag gtgaacatgg ataaggatat caatctgaat
      961 aacaacaata acattaagaa aaagaacaat aacgatattg actgtgattg cgtcgaccag
     1021 aaaatgaaca accatgtcaa caacaaaaac tacatcaact ctttccgcga cccgcaggaa
     1081 atcattaaca atatggtcga caatatcgag ttcttgagca tcccgcacgt ttataccacc
     1141 catcgttact cgatcttgct gtgcttgaac gacatgattc cagaatacaa gcacaatgtt
     1201 ctgaacaaca ccattcgctg tttgtacaac aagtatgtta gccgtatgaa agagcagtac
     1261 aacattaaca tcaaagagaa taacaaacgc atttatgtgc tgaaggaccg tatcagctac
     1321 ttgaaagaga aaaccaacat tgtcggcatc ttgaacgtca actatgacag cttcagcgat
     1381 ggcggcattt tcgttgagcc gaaacgtgca gttcagcgta tgtttgaaat gatcaacgag
     1441 ggcgcgagcg tgattgacat tggcggtgag tccagcggtc cgtttgtcat tccgaacccg
     1501 aaaatcagcg agcgcgacct ggttgttccg gtcctgcaac tgtttcagaa agagtggaat
     1561 gatatcaaga acaagattgt caagtgtgat gcgaaaccga ttatctccat cgataccatc
     1621 aactacaatg tgtttaaaga atgcgtggac aatgatctgg ttgacatcct gaatgacatc
     1681 agcgcgtgta ccaataaccc agagatcatc aagctgctga aaaagaaaaa caagttctat
     1741 agcgttgtct tgatgcacaa gcgtggtaat cctcatacca tggacaagct gaccaattat
     1801 gataatctgg tttacgacat taagaattac ctggaacagc gcctgaattt cctggtgctg
     1861 aacggtatcc cgcgttaccg tattctgttt gacatcggtc tgggtttcgc caagaagcac
     1921 gaccaatcca ttaagctgct gcagaacatc catgtgtatg acgagtatcc gctgttcatc
     1981 ggttatagcc gcaagcgctt tatcgcacac tgcatgaacg atcaaaatgt tgtgattaac
     2041 acgcaacaaa agctgcacga tgagcagcag aacgagaaca agaacatcgt ggataagagc
     2101 cacaactgga tgttccaaat gaattacatg cgtaaggata aagatcagct gctgtatcaa
     2161 aagaacattt gtggtggctt ggctattgca agctacagct attacaagaa agtggacctg
     2221 atccgtgttc acgacgttct ggaaacgaag tctgtgctgg acgttctgac caagattgac
     2281 caggttcacc accatcacca tcattaactc agaccccaag ggcgacacaa aatttattct
     2341 aaatgataat aaatactgat aacatcttat agtttgtatt atattttgta ttatcgttga
     2401 catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag atttattttc
     2461 ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga
     2521 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt
     2581 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacaggcgcc
     2641 cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa cccgccgaag
     2701 cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc ccagggggcc
     2761 cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa cgcaaaaagg
     2821 ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact ctcgccttcc
     2881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtgggctaac
     3481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3661 ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt
     3721 tggtcatgag tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     3841 tcaccagtga gactggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     3901 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     3961 gcgggatata acatgagcta tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     4021 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     4081 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     4141 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     4201 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     4261 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     4321 cctcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     4381 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     4441 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     4501 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     4561 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     4621 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     4681 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     4741 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     4801 ttactggttt catattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     4861 cgcgaaaggt tttgcgccat tcgatggcgc gccgcttacc aatgcttaat cagtgaggca
     4921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4981 ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat accgcgagaa
     5041 ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc tacaggcatc
     5221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5461 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5521 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5581 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5641 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5701 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatattc
     5761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5821 tttgaatgta tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa ttaaccaatt
     5881 ctgaacatta tcgcgagccc atttatacct gaatatggct cataacaccc cttgtttgcc
     5941 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     6001 tagcgccgat ggtagtgtgg ggactcccca tgcgagagta gggaactgcc aggcatcaaa
     6061 taaaacgaaa ggctcagtcg aaagactggg cctttcgccc gggctaatta tggggtgtcg
     6121 ccctt
//
