LOCUS       sequence-123747-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E17TAG GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: GFP E17TAG
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..57)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(64..777)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILV*LDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(104..124)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     RBS             784..795
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tagcagccgg atcaagcttt gaattgagct cgcccttcag tggtggtggt ggtggtgctc
       61 gagtttgtag agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag
      121 gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta
      181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      241 tgctagttga acggatccat cttcaatgtt gtggcgaatt ttgaagttag ctttgattcc
      301 attcttttgt ttgtctgccg tgatgtatac attgtgtgag ttatagttgt attcgagttt
      361 gtgtccgaga atgtttccat cttctttaaa atcaatacct tttaactcga tacgattaac
      421 aagggtatca ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa
      481 agatatagtg cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      541 tttcatgtga tccggataac gggaaaagca ttgaacacca taagagaaag tagtgacaag
      601 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
      661 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      721 atctaactaa acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcatggt
      781 acctttctcc tcttt
//
