LOCUS       sequence-123752-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E17/Q157 TAG GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: GFP E17TAG/Q157TAG
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(66..779)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILV*LDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADK*KNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(106..126)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     RBS             786..797
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gttagcagcc ggatcaagct ttgaattgag ctcgcccttc agtggtggtg gtggtggtgc
       61 tcgagtttgt agagctcatc catgccatgt gtaatcccag cagcagttac aaactcaaga
      121 aggaccatgt ggtcacgctt ttcgttggga tctttcgaaa gggcagattg tgtcgacagg
      181 taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg
      241 tctgctagtt gaacggatcc atcttcaatg ttgtggcgaa ttttgaagtt agctttgatt
      301 ccattcttct atttgtctgc cgtgatgtat acattgtgtg agttatagtt gtattcgagt
      361 ttgtgtccga gaatgtttcc atcttcttta aaatcaatac cttttaactc gatacgatta
      421 acaagggtat caccttcaaa cttgacttca gcacgcgtct tgtagttccc gtcatctttg
      481 aaagatatag tgcgttcctg tacataacct tcgggcatgg cactcttgaa aaagtcatgc
      541 cgtttcatgt gatccggata acgggaaaag cattgaacac cataagagaa agtagtgaca
      601 agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag ggtaagtttt
      661 ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc attaacatca
      721 ccatctaact aaacaagaat tgggacaact ccagtgaaaa gttcttctcc tttactcatg
      781 gtacctttct cctctttaa
//
