LOCUS       sequence-123754-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phosphoserine orthogonal translation system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SepOTS-beta
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        473..550
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(570..589)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atatggtatt gataatcctg atatgagtaa attgcagttt catttgatgc tcgatgagtt
       61 tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      121 taggggttcc gcgcacattt ccccgaaaag tgccaccgcg gccgcaaaaa aaatccttag
      181 ctttcgctaa ggatctgcag gtcgactcta gaggatcctg gagccggggg tgggatttga
      241 acccacgtaa ggcggatcta gagtccgctg cctaacccct agactacccc ggcgaattca
      301 gcgttacaag tattacacaa agttttttat gttgagaata tttttttgat ggggcgccac
      361 ttatttttga tcgttcgctc aaagaagcgc ggccgctttc accgtcatca ccgaaacgcg
      421 cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat
      481 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
      541 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      601 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      661 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      721 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      781 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      841 gatggtagaa cgaagcggcg tc
//
