LOCUS       sequence-123755-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phosphoserine orthogonal translation system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SepOTS-gamma
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        537..614
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(634..653)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tatggtattg ataatcctga tatgagtaaa ttgcagtttc atttgatgct cgatgagttt
       61 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      121 aggggttccg cgcacatttc cccgaaaagt gccaccgcgg ccgccctgtt gcccgtctca
      181 ctggtgaaaa gaaaaaccac cctggcgccg cttctttgag cgaacgatca aaaataagtg
      241 gcgccccatc aaaaaaatat tctcaacata aaaaactttg tgtaatactt gtaacgctga
      301 attcgccggg gtagtctagg ggttaggcag cggactctag atccgcctta cgtgggttca
      361 aatcccaccc ccggctccag gatcctctag agtcgacctg cagatcctta gcgaaagcta
      421 aggatttttt ttaagcttgg cactggccgt cggcggccgc tttcaccgtc atcaccgaaa
      481 cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca
      541 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
      601 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
      661 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
      721 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
      781 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
      841 cgtcgcaaat tgtcgcggcg attaaatctc gcgccg
//
