LOCUS       sequence-123758-         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas 
            fluorescens, codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_N300D
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgaggctacc aacaggtttt ttcagcagat
       61 gtttcagggt ggtggtaaca ccattatcac gcaggctacc ataacaacgt tcaaatgcgg
      121 caacaaattc cggactattc ggaattgcgg tgccaaaaat ttcttcaact gccagcagac
      181 gctggctaat cagtgcatca tctgaaacca gaccctgaca aaattctgca cgcggatcag
      241 gaatggtata gctcacacca ttttcatcaa cgcctttcag atacagtgcc catgctgcaa
      301 caaccagtgc tgcacgttcg gtttcacgac catctgcaat caaacgatta attgtcggaa
      361 cggtaaattt aggaaatttg ctgctaccat cactacaaac acgttccagc tgatctgcaa
      421 ttgcctgatt gctaaaacga tcaacca
//
