LOCUS       sequence-123760-        4344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses M2-SNX17 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pECE M2-SNX17 WT
FEATURES             Location/Qualifiers
     source          1..4344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(153..172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     207..226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(309..328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(587..604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(758..1346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(838..857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1518..2309,2310..2378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2141..2160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2379..2483)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2513..2704
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2536..2556)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2554..2851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2893..2916
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taagtaatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtgcggtgt gaaataccgc
      541 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
      601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
      961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaaag tatatatgag
     1501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1801 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     1861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2101 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ttctgaggcg
     2521 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2701 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2761 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2821 tattccagaa gtagtgagga ggcttttttg gaggagatct aagcttggta ccgagctcgg
     2881 atcctcagga tggactacaa ggacgatgac gataaacccg ggaattcgca cttttccatt
     2941 cccgaaaccg agtcccgcag cggggacagc ggcggctccg cctacgtggc ctataacatt
     3001 cacgtgaatg gagtcctgca ctgtcgggtg cgctacagcc agctcctggg gctgcacgag
     3061 cagcttcgga aggagtatgg ggccaatgtg cttcctgcat tccccccaaa gaagcttttc
     3121 tctctgactc ctgctgaggt agaacagagg agagagcagt tagagaagta catgcaagct
     3181 gttcggcaag acccattgct tgggagcagc gagactttca acagtttcct gcgtcgggca
     3241 caacaggaga cacagcaggt ccccacagag gaagtgtcct tggaagtgct gctcagcaac
     3301 gggcagaaag ttctggtcaa cgtgctaact tcagatcaga ctgaggatgt cctggaggct
     3361 gtagctgcaa agctggatct tccagatgac ttgattggat actttagtct attcttagtt
     3421 cgagaaaaag aggatggagc cttttctttt gtacggaagt tgcaagagtt tgagctgcct
     3481 tatgtgtctg tcaccagcct tcggagtcaa gagtataaga ttgtgctaag gaagagttat
     3541 tgggactctg cctatgatga cgatgtcatg gagaaccggg ttggcctgaa cctgctttat
     3601 gctcagacgg tatcagatat tgagcgtggg tggatcttgg tcaccaagga acagcaccgg
     3661 caactcaaat ctctgcaaga gaaagtctcc aagaaggagt tcctgagact ggcccagacg
     3721 ctgcggcact atggctactt gcgctttgat gcctgtgtgg ctgacttccc agaaaaggac
     3781 tgtcctgtgg tggtgagcgc gggcaacagt gagctcagcc tgcagctccg cctgcctggc
     3841 cagcaactcc gagaaggctc cttccgggtc acccgcatgc gatgctggcg ggtcacctcc
     3901 tctgtaccat tgcccagtgg aagcacgagc agcccaggcc ggggccgggg tgaggtgcgc
     3961 ctggaactgg cttttgaata cctcatgagc aaggaccggc tacagtgggt caccatcact
     4021 agcccccagg ctatcatgat gagcatctgc ttgcagtcca tggttgatga actgatggtg
     4081 aagaaatctg gcggcagtat caggaagatg ctgcgccggc gggtgggggg tactctgaga
     4141 cgctcagaca gccagcaagc agtgaagtcc ccaccactgc ttgagtcacc tgatgccacc
     4201 cgggagtcta tggtcaaact ctcaagtaag ctgagtgccg tgagcttgcg gggaattggc
     4261 agtcccagca cagatgccag tgccagtgat gtccacggca atttcgcctt cgagggcatt
     4321 ggagatgagg atctgtaatc taga
//
