LOCUS       sequence-123768-        5030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for Calb2 promoter (-161to +80, mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Kresoja-Rakic J, Kapaklikaya E, Ziltener G, Dalcher D, Santoro R, 
            Christensen BC, Johnson KC, Schwaller B, Weder W, Stahel RA, 
            Felley-Bosco E
  TITLE     Identification of cis- and trans-acting elements regulating 
            calretinin expression in mesothelioma cells.
  JOURNAL   Oncotarget. 2016 Feb 1. doi: 10.18632/oncotarget.7114.
  PUBMED    26848772
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2016 Feb 1. doi: 10.18632/oncotarget.7114."
COMMENT     Sequence Label: -161+80pCalb2/mutE2F(-69)-like
FEATURES             Location/Qualifiers
     source          1..5030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             300..1952
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(366..384)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1993..2114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2004..2023)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2058..2077
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2362..2379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2533..3121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2613..2632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3292..4083,4084..4152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3915..3934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4153..4257)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4284..4739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4371..4390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4581..4602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4870..4918
                     /note="synthetic polyadenylation signal"
     misc_feature    4932..5023
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4972..4991
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagctttc
       61 tcagcgcaga ggtaagggcc ctctaggagt ccgggccgag cccctcgcgc cgccgccccc
      121 gccgcgccgc gccccggtcg gcttccctga attcgcgcgc ccccttctgg cggccgggcg
      181 caggcgcagg ctccagagcg tatataaggg cagcgtggcg cacaacccca gcgcgagtgc
      241 cagagcccag ccggcgcgga gcgggagcgg tgcaggctga ggtctccgag cggctcgcca
      301 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      361 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      421 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      481 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      541 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      601 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
      661 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
      721 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
      781 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
      841 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
      901 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
      961 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     1021 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     1081 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     1141 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     1201 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     1261 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     1321 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     1381 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     1441 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     1501 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     1561 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     1621 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     1681 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     1741 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     1801 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     1861 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     1921 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
     1981 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2041 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2101 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2161 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2221 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2281 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2341 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2701 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2941 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4261 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4621 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4681 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     4741 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4801 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg
     4861 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     4921 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     4981 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
//
