LOCUS       sequence-123772-        5255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for Calb2 promoter (-419 to +80, WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Kresoja-Rakic J, Kapaklikaya E, Ziltener G, Dalcher D, Santoro R, 
            Christensen BC, Johnson KC, Schwaller B, Weder W, Stahel RA, 
            Felley-Bosco E
  TITLE     Identification of cis- and trans-acting elements regulating 
            calretinin expression in mesothelioma cells.
  JOURNAL   Oncotarget. 2016 Feb 1. doi: 10.18632/oncotarget.7114.
  PUBMED    26848772
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2016 Feb 1. doi: 10.18632/oncotarget.7114."
COMMENT     Sequence Label: pGL3-B-419;+80pCalb2
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             525..2177
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(591..609)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2218..2339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2229..2248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2283..2302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2587..2604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2758..3346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2838..2857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3517..4308,4309..4377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4140..4159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4378..4482)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4509..4964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4596..4615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4806..4827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5095..5143
                     /note="synthetic polyadenylation signal"
     misc_feature    5157..5248
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5197..5216
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcagct cttcctgctg tggaaggatc agaagagagg
       61 aggcacgaca gagacctagt gaactccgaa gcccgagtgc taaatatttg tcaagtttgt
      121 gttagtatta ctattagtgt tgttactgct gttattatta ttgctactgc cagcaataat
      181 aagtggtgga tgtactcaag acggtcggga gggaaggcaa gggcagcctc tccctcattt
      241 tcaccgaaaa tcctccgggt gtccctggcc ccgcgccgag gggtctcagc gcagaggtaa
      301 gggccctcta ggagtccggg ccgagcctct cgcgccgccg cccccgccgc gccgcgcccc
      361 ggtcggattc cctgagcgcg cgcgccccct tctggcggcc gggcgcaggc gcaggctcca
      421 gagcgtatat aagggcagcg tggcgcacaa ccccagcgcg agtgccagag cccagccggc
      481 gcggagcggg agcggtgcag gctgaggtct ccgagcggct cgccatggaa gacgccaaaa
      541 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
      601 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      661 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
      721 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      781 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
      841 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
      901 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
      961 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     1021 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
     1081 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
     1141 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
     1201 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     1261 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     1321 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     1381 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     1441 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     1501 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     1561 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     1621 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     1681 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     1741 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     1801 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     1861 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     1921 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     1981 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     2041 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     2101 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     2161 gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     2221 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2281 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2341 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2401 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
     2461 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     2521 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     2581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2941 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4501 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4861 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4921 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag
     4981 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa
     5041 gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa
     5101 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     5161 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     5221 gcaagtgcag gtgccagaac atttctctat cgata
//
