LOCUS       sequence-123783-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd mChO intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU12
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             306..326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(762..795)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gcatgctcga gcggccgcca gtgtgatgga tatctgcaga attcgccctt ggctgtctca
       61 tcctactttc acctagttaa ctgcttgtct taaaatctat gcttctcttt agtatctaaa
      121 attttcctag aagcttacaa gtatataaat ggtctcttct caataaaggt tgtatattta
      181 ttcatcttat tgaatctgcc atttcctcgt ttttgcgagt ttatatacct tccaattttc
      241 tttctattgt attttcaact tctaatttta attcagggaa actgcttcaa cgcatcatga
      301 ccgctccaaa gaagaaacgc aaagtaccgg tagaaaaaat ggtctcaaag ggtgaagaag
      361 ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag ggatctgtca
      421 atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag ggaacacaaa
      481 ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
      541 ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct ccacagttca
      601 tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat ttgaaacttt
      661 cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga ggagttgtta
      721 cagtgacgca agactcaagg taagtttaaa cagttcggta cgaagttcct atactttcta
      781 gagaatagga acttccctgt tgacaattaa tcatcggcat agtatatcgg cata
//
